论文部分内容阅读
目的 克隆和表达日本血吸虫 (Schistosomajaponicum ,Sj)CAI基因 ,并对表达产物进行免疫保护效果测定 ,评价其作为血吸虫疫苗候选分子的潜能。 方法 将SjCAI基因亚克隆至pGEX 5X 3载体 ,转化入感受态大肠杆菌ER2 56 6 ,在异丙基 β D 硫代半乳糖苷 (IPTG)诱导下进行表达 ,用表达产物免疫小鼠 ,并设分别注射等体积的弗氏完全佐剂和PBS的两组对照组 ,观察免疫保护效果。 结果 在IPTG诱导下 ,表达载体中的SjGST基因与重组的SjCAI基因在大肠杆菌中获得了高效融合表达 ,将其免疫小鼠 ,诱导产生了 2 9.87%的减虫率和 6 3.71 %的减卵率。 结论 SjCAI基因亚克隆至pGEX 5X 3载体后可在大肠杆菌中高效表达 ,表达产物能诱导产生一定水平的抗Sj保护性免疫力
Objective To clone and express the CAI gene of Schistosoma japonicum (Sj) and evaluate the immunoprotective effect of the expression product on the expression of Schistosoma japonicum (Sj) as a candidate molecule for schistosoma vaccine. Methods The SjCAI gene was subcloned into pGEX 5X 3 vector, transformed into competent E. coli ER2 566, expressed under the induction of isopropyl β D thiogalactoside (IPTG), and the mice were immunized with the expressed product The same volume of Freund’s complete adjuvant and PBS were injected into two groups respectively to observe the protective effect of immunization. Results Under the induction of IPTG, the SjGST gene in the expression vector and the recombinant SjCAI gene were highly expressed in Escherichia coli, and the mice were immunized to induce the worm reduction rate of 2 9.87% and the egg reduction rate of 6 3.71% rate. Conclusion The SjCAI gene is highly expressed in E. coli after it is subcloned into pGEX 5X 3 vector and the expressed product can induce a certain level of protective immunity against Sj