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以DUS测试指南中规定的49个水稻标准品种为材料,根据2008年在杭州田间试验对59个形态性状的调查数据,利用PopGen32软件计算了等位变异数、有效等位变异数和Shannon’s多样性信息指数,对标准品种的形态性状多样性进行了评价;并根据其多样性参数采用NTSYS软件对标准品种进行了聚类分析。结果表明,59个形态性状在19份籼稻和30份粳稻品种中分别检测到201和262个等位变异,平均每个性状分别检测到3.4068和4.4407个等位变异,籼、粳稻类群中59个性状的平均Shannon’s多样性指数分别为0.8318(0～1.7674)和0.9828(0～1.8547)。聚类分析结果表明,籼、粳稻群体内品种间的形态性状相似系数分别介于0.45～0.81和0.36～0.76,取相似系数0.70为阈值,可将19份籼稻和30份粳稻品种分别分为16和27类。多样性分析表明本研究的49个品种形态性状多样性丰富,具有标准品种性状描述的示例和校正作用;根据聚类分析结果,可将现有标准品种的数量减少至43个。
Based on the survey data of 59 morphological traits in field experiments of Hangzhou in 2008, 49 rice standard varieties specified in the DUS test guideline were used to calculate alleles, effective alleles and Shannon’s diversity using PopGen32 software Information index, the diversity of morphological traits of standard varieties were evaluated. According to their diversity parameters, the standard varieties were clustered by NTSYS software. The results showed that 201 and 262 alleles were detected in 19 indica rice varieties and 30 japonica rice varieties, respectively. An average of 3.4068 and 4.4407 alleles were detected in each of the 59 morphological traits, while 59 The average Shannon’s diversity index of traits was 0.8318 (0 ~ 1.7674) and 0.9828 (0 ~ 1.8547), respectively. Cluster analysis showed that the similarity coefficients of morphological traits among indica and japonica populations ranged from 0.45 to 0.81 and 0.36 to 0.76, respectively, and the similarity coefficient of 0.70 was the threshold value. 19 indica and 30 japonica cultivars could be divided into 16 And 27 categories. The diversity analysis showed that the morphological traits of 49 cultivars in this study were rich in diversity, with the examples and corrections of the traits of standard cultivars. According to the results of cluster analysis, the number of existing standard cultivars could be reduced to 43.