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为了实现分子标记或基因辅助育种在牙鲆养殖中成功运用,并快速提高牙鲆产量,使用微卫星标记首次构建了国内第一张牙鲆遗传连锁图谱。采用基因组测序的方法,筛选出大量微卫星序列,从中随机挑选1 000条序列设计合成引物,利用2009年建立的第10号家系为作图群体,使用JoinMap4.0软件,构建了牙鲆(Paralichthys olivaceus)SSR标记遗传连锁图谱。雌雄图谱分别由24个连锁群组成,其中雌性图谱标记212个,总长度1 320.4 cM,覆盖率为77.7%;雄性图谱标记198个,总长度1 361 cM,覆盖率为76.1%。每个连锁群长度变动在9.3～116.1 cM之间,连锁群上的标记数在3～21个之间。各连锁群上的SSR标记并不是均匀分布的,其中1、3和8号连锁群存在标记密集区。该图谱能够进行初步的QTL定位分析和基因定位相关研究,为开展牙鲆基因和QTL的精细定位及分子标记辅助育种(MAS)等提供更有效的依据。
In order to achieve the successful application of molecular marker or gene-assisted breeding in Japanese flounder breeding and rapidly increase the production of Japanese flounder, the first linkage map of Japanese flounder was established by using microsatellite markers. A large number of microsatellite loci were screened by using the method of genome sequencing. One thousand sequences were randomly selected to design and synthesize the primers. Based on the No. 10 family established in 2009, JoinMap4.0 software was used to construct Paralichthys olivaceus) SSR marker genetic linkage map. The male and female map were composed of 24 linkage groups, of which 212 were female, with a total length of 1 320.4 cM, with a coverage rate of 77.7% and 198 male markers with a total length of 1 361 cM and a coverage rate of 76.1%. The variation of length of each linkage group was between 9.3 and 116.1 cM, and the number of markers on the linkage group ranged from 3 to 21. The SSR markers on each linkage group were not evenly distributed, of which the linkage groups 1, 3 and 8 were marked with marker-intensive regions. The map can be used for preliminary QTL mapping and gene mapping, providing a more effective basis for fine mapping of QTLs and molecular marker-assisted breeding (MAS) of flounder.