论文部分内容阅读
目的通过检测胰腺腺泡细胞核转录因子(NF-κB)活性及血液IL-2、IL-6、IL-10、TNFα及ICAM-1含量的变化,探讨吡咯烷二硫代氨基甲酸脂(PDTC)对重症急性胰腺炎(SAP)胰腺腺泡细胞NF-κB活性和血液炎症细胞因子的影响。方法采用5%牛磺胆酸钠胰管逆行注射制备SAP动物模型。SD雄性大鼠40只,按随机分配原则分为SAP(+)PDTC治疗组和SAP(-)空白对照组。采用EMSA法检测胰腺腺泡细胞胞核NF-κB活性、Western-blotting法检测胰腺腺泡细胞胞质IκBa抑制活性及ELISA法检测血液IL-2、IL-6、IL-10、TNFα及ICAM-1含量。结果PDTC在1h、3h、5h及7h均可显著抑制SAP胰腺腺泡细胞胞核NF-κB活性(22.47±5.39 vs 31.36±5.72、27.92±4.75 vs 39.44±6.31、23.77±3.95 vs 33.80±5.96及19.78±3.48 vs 25.69±4.91)(P<0.01);显著增强SAP胰腺腺泡细胞胞质IκBa活性(8.55±1.26 vs 6.37±1.19、7.31±1.36 vs 5.91±1.65、9.53±1.73 vs 6.85±1.37及9.19±1.48 vs .97±0.86)(P<0.01);显著抑制血液中炎症细胞因子IL-2、IL-6、IL-10、TNFα及ICAM-1活性(P<0.05)。结论吡咯烷二硫代氨基甲酸酯可通过抑制SAP胰腺腺泡细胞NF-κB活性,增加胰腺腺泡细胞胞质IκBa抑制活性的能力,减少血液中炎症细胞因子活性,从而抑制SAP引起的过度炎症反应。
Objective To investigate the effects of pyrrolidine dithiocarbamate (PDTC) on pancreatic acinar cell nuclear factor-kappa B (NF-κB) and blood levels of IL-2, IL-6, IL-10, TNFα and ICAM- On acute pancreatitis (SAP) pancreatic acinar cells NF-κB activity and blood inflammatory cytokines. Methods The animal model of SAP was established by retrograde injection of 5% sodium taurocholate pancreatic duct. Forty male Sprague-Dawley rats were randomly divided into two groups: SAP (+) PDTC treatment group and SAP (-) blank control group. The activity of NF-κB in the nuclei of pancreatic acinar cells was detected by EMSA, the IκBa inhibitory activity of pancreatic acinar cells was detected by Western-blotting and the levels of IL-2, IL-6, IL-10, TNFα and ICAM- 1 content. Results PDTC significantly inhibited the nuclear NF-κB activity in pancreatic acinar cells at 1h, 3h, 5h and 7h (22.47 ± 5.39 vs 31.36 ± 5.72, 27.92 ± 4.75 vs 39.44 ± 6.31, 23.77 ± 3.95 vs 33.80 ± 5.96 and 19.78 ± 3.48 vs 25.69 ± 4.91) (P <0.01). The cytosolic IκBa activity of pancreatic acinar cells was significantly increased (8.55 ± 1.26 vs 6.37 ± 1.19, 7.31 ± 1.36 vs 5.91 ± 1.65, 9.53 ± 1.73 vs 6.85 ± 1.37 vs 9.19 ± 1.48 vs. 97 ± 0.86) (P <0.01). The activities of IL-2, IL-6, IL-10, TNFα and ICAM-1 in blood were significantly decreased (P <0.05). Conclusion Pyrrolidine dithiocarbamate can inhibit SAP-induced hyperphosphorylation by inhibiting the activity of NF-κB in pancreatic acinar cells and increasing the inhibitory activity of cytosolic IκBa in pancreatic acinar cells, reducing the activity of inflammatory cytokines in the blood Inflammation.