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目的:溃疡性结肠炎病因和发病机制目前尚不明确,抗炎与促炎因子的失衡可能在UC的发生发展中起到一定的作用。卵泡抑素-1是一种具有广泛糖基化修饰的分泌糖蛋白,目前的研究倾向于是一种炎性蛋白。本研究检测溃疡性结肠炎小鼠模型结肠标本中FSTL1的表达,分析探讨FSTL1在溃疡性结肠炎发病的中的作用。方法:20只BALB/c小鼠均分为对照组、模型组。模型组予以4%DSS喂养一周,对照组予以普通饮水一周,监测小鼠疾病症状,对小鼠疾病活动指数DAI进行评分。观察FSTL1在结肠黏膜组织中的表达,检测结肠组织FSTL1蛋白及FSTL1 mRNA表达水平,对FSTL1表达与小鼠疾病活动指数DAI进行相关性分析。采用t检验及Pearson相关分析,进行统计分析。结果:HE染色显示模型组病变主要累及黏膜及黏膜下层,可见大量炎性细胞浸润,以中性粒细胞为主,部分表面上皮脱落,上皮内杯状细胞减少,隐窝破坏;FSTL1蛋白表达于肠黏膜腺体间质,模型组表达高于正常对照组,分别为(2.9±1.44)和(0.6±0.51),具有显著性差异,P<0.0l;实验组结肠FSTL1 mRNA平均表达水平(1.57±0.23)较对照组(0.46±0.22)明显增加,(t=10.84,P<0.05)。结肠组织FSTL1mRNA表达水平与小鼠DAI成正相关,相关系数为r=0.850,P<0.05。结论:研究中发现溃疡性结肠炎小鼠模型中FSTL1表达较正常对照组明显升高,且与小鼠DAI成正相关。提示FSTL1可能与实验性结肠炎的发生发展有一定的相关性,可能可以作为预测溃疡性结肠炎活动度的新的炎性标记物应用。
OBJECTIVE: The etiology and pathogenesis of ulcerative colitis are not yet clear. The imbalance between anti-inflammatory and proinflammatory cytokines may play a role in the occurrence and development of UC. Follistatin-1 is a secreted glycoprotein with extensive glycosylation and current research tends to be an inflammatory protein. This study was to detect the expression of FSTL1 in colonic mucosa of ulcerative colitis mouse model and to explore the role of FSTL1 in the pathogenesis of ulcerative colitis. Methods: Twenty BALB / c mice were divided into control group and model group. The model group was fed with 4% DSS for one week while the control group was given normal drinking water for one week. The symptoms of the mice were monitored and the disease activity index DAI of the mice was scored. To observe the expression of FSTL1 in colonic mucosa, to detect the expression of FSTL1 protein and FSTL1 mRNA in colon tissue, and to analyze the correlation between FSTL1 expression and disease index DAI in mice. Using t-test and Pearson correlation analysis, statistical analysis. Results: HE staining showed that the lesions in the model group mainly involved the mucosa and submucosa. A large number of infiltrative inflammatory cells were seen in the model group, mainly neutrophils, partial epithelial shedding, goblet cells in the epithelium and destruction of crypts. The expression of FSTL1 protein in The expression of FSTL1 mRNA in the experimental group was significantly higher than that in the normal control group (2.9 ± 1.44 vs 0.6 ± 0.51, P <0.01) ± 0.23) than the control group (0.46 ± 0.22) (t = 10.84, P <0.05). The expression of FSTL1 mRNA in colon tissue was positively correlated with DAI in mice, the correlation coefficient was r = 0.850, P <0.05. Conclusion: In the study, FSTL1 expression in the ulcerative colitis mouse model was significantly higher than that in the normal control group and positively correlated with the DAI in mice. These results suggest that FSTL1 may be associated with the development of experimental colitis and may be used as a new inflammatory marker to predict the activity of ulcerative colitis.