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测定慢性非透析肾病患者红细胞内碳酸酐酶I、碳酸酐酶Ⅱ的浓度,并观察其与酸毒症、微量元素锌、贫血及补铁的关系。用酶联免疫吸附反应法测定38名非透析肾病患者红细胞内碳酸酐酶的浓度,原子吸收分光光度法测定锌的浓度。研究结果显示,患者体内碳酸酐酶水平升高,平均红细胞内锌浓度也升高,而血浆中锌浓度在临界值。碳酸酐酶I和红细胞锌呈显著正相关,与铁蛋白、pH值和重碳酸盐无显著相关。补铁后,碳酸酐酶水平不改变,但红细胞中锌降低。由此推测,慢性肾病患者红细胞中碳酸酐酶增加,但这不能被缺铁或酸毒症解释。
The levels of erythrocyte carbonic anhydrase I and carbonic anhydrase II in patients with chronic non-dialysis nephropathy were measured and the relationship with acidosis, trace elements zinc, anemia and iron supplementation was observed. Enzyme linked immunosorbent assay was used to determine erythrocyte carbonic anhydrase concentration in 38 non-dialysis nephropathy patients and zinc concentration was determined by atomic absorption spectrophotometry. The results show that patients with elevated levels of carbonic anhydrase, the average intracellular zinc concentration also increased, while the plasma concentration of zinc at the critical value. There was a significant positive correlation between carbonic anhydrase I and erythrocyte zinc, but not with ferritin, pH and bicarbonate. After iron supplementation, carbonic anhydrase levels did not change but zinc in erythrocytes decreased. This suggests that carbonic anhydrase in erythrocytes increases in patients with chronic kidney disease, but this can not be explained by iron deficiency or acidosis.