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目的探讨脑动脉细胞间缝隙连接在高血压病中的变化。方法应用全细胞膜片钳、定量RT-PCR和Western blot技术,比较自发性高血压(SH)大鼠(n=30)和Wistar大鼠(n=30)脑动脉上缝隙连接耦联力,以及连接蛋白45(Cx45)在mRNA和蛋白水平的表达。结果①应用全细胞膜片钳技术发现,SH大鼠脑动脉平滑肌细胞膜电容和膜电导都明显高于正常Wistar大鼠(P<0.05),提示SH大鼠脑动脉平滑肌细胞间的缝隙连接耦联力增强。②应用定量RT-PCR技术发现,SH大鼠脑动脉Cx45 mRNA的表达升高(P<0.05)。③应用Western blot发现,SH大鼠脑动脉Cx45的蛋白表达升高(P<0.01)。结论 SH大鼠可能通过上调平滑肌细胞上Cx45的表达,增强脑动脉平滑肌细胞间缝隙连接通讯,保证血管的同步收缩。
Objective To investigate the changes of interphalangeal connections of cerebral arteries in hypertensive patients. Methods Whole-cell patch clamp, quantitative RT-PCR and Western blot were used to compare the gap junctional coupling force in the cerebral arteries of spontaneously hypertensive rats (n = 30) and Wistar rats (n = 30) Connexin 45 (Cx45) expression at mRNA and protein levels. Results ① Using whole-cell patch-clamp technique, it was found that the membrane capacitance and membrane conductance of cerebral artery smooth muscle cells in SH rats were significantly higher than those in normal Wistar rats (P <0.05), suggesting that the gap junctional connections Enhanced. (2) The expression of Cx45 mRNA in SH rat arteries was increased by quantitative RT-PCR (P <0.05). ③ Western blot showed that the protein expression of Cx45 in SH rat arteries increased (P <0.01). Conclusion SH rats may increase the Cx45 expression on smooth muscle cells and enhance the gap junctional communication between smooth muscle cells of cerebral arteries to ensure the synchronous contraction of blood vessels.