论文部分内容阅读
目的了解赤峰市阿鲁科尔沁旗达乌尔黄鼠鼠疫疫源地内鼠类、家畜家禽携带小肠结肠炎耶尔森菌情况,为疾病防治工作和研究鼠疫疫源地提供科学依据。方法在达乌尔黄鼠鼠疫疫源地内,选择以村庄为中心,向外依次为农田、草原的同心圆样地,分别采集鼠类、家畜家禽粪便、舌根、咽拭子及肠道内容物等样品,进行小肠结肠炎耶尔森菌分离、培养、鉴定、生物血清分型,并用PCR方法进行毒力因子测定。统计学分析采用χ2检验。结果 2009-2012年共检验各类样品3837份,检出小肠结肠炎耶尔森菌17株,总检出率为0.44%。2011年检出的16株小肠结肠炎耶尔森菌中13株源自猪粪,3株来自鼠肠;1株O∶3血清型,生物3型,携带ail、yst A、rfb C毒力基因的致病性菌株检自猪粪。结论阿鲁科尔沁旗绍根地区的猪、鼠体内携带小肠结肠炎耶尔森菌,猪是致病性小肠结肠炎耶尔森菌的重要携带者。进一步调查分析小肠结肠炎耶尔森菌在该地区人和宿主动物间的分布规律十分必要。
Objective To understand the status of Yersinia enterocolitica in mice and livestock in the plague foci of Darusschrim rats in Aruqerqin Banner of Chifeng City and to provide scientific basis for disease prevention and control and plague foci. Methods In the plague foci of Daurian ground squirrel, the concentric circles of farmland and grassland were chosen as the center and the feces, tongue base, throat swab and intestinal contents Yersinia enterocolitica isolates, culture, identification, biological serum typing, and PCR method for virulence factor determination. Statistical analysis using χ2 test. Results A total of 3837 samples of various types were tested from 2009 to 2012. 17 strains of Yersinia enterocolitica were detected with a total detection rate of 0.44%. Of the 16 strains of Yersinia enterocolitica detected in 2011, 13 were from swine manure and 3 were from the murine intestine; 1 strain was O: 3 serotype 3 and was biotype 3 carrying ail, yst A and rfb C virulence Pathogenic strains of the gene are seized from pig manure. Conclusion The pigs and mice in the Shorgen region of Aruqerqin Banner carry Yersinia enterocolitica in vivo, and the pigs are important carriers of pathogenic Yersinia enterocolitica. Further investigation and analysis of Yersinia enterocolitica in the region between the distribution of human and host animals is very necessary.