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目的 探讨人参皂甙对脊髓损伤的作用 ,寻求治疗脊髓损伤的新方法。方法 采用改良 Allen氏重量打击法制作猫脊髓损伤模型。动物随机分组 ,通过生物化学及病理学手段检测急性脊髓损伤中脊髓损伤节段水、钙离子 (Ca2 + )、丙二醛 (MDA)含量 ,过氧化物歧化酶 (SOD)、谷胱苷肽过氧化物酶 (GSH)活性 ,观察伤段脊髓光境下组织形态改变及人参皂甙对其的影响。结果 1损伤组伤段脊髓水、Ca2 + 、MDA含量明显升高 ,SOD、GSH活性明显降低 (P<0 .0 )。 2光镜下各组均有水肿 ,损伤组最重、治疗组次之、对照组最轻 ;损伤组及治疗组均有中心性出血 ,损伤组较重 ;损伤组 3h即出现神经元空泡变性 ,核溶解或固缩 ,尼氏小体消失 ;6h出现部分神经纤维脱髓鞘或断裂。治疗组均有不同程度的恢复。结论 人参皂甙对脊髓损伤早期有保护作用。
Objective To investigate the effect of ginsenosides on spinal cord injury and seek new methods for treating spinal cord injury. Methods The modified Allen’s weight hit method was used to make a cat spinal cord injury model. Animals were randomly divided into groups to detect levels of water, calcium (Ca2 +), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione in the spinal cord of acute spinal cord injury by biochemical and pathological methods. Peroxidase (GSH) activity was observed to observe the histopathological changes in the injured spinal cord and the effects of ginsenosides. Results 1 In the injury group, the content of water, Ca2+, MDA in the spinal cord was significantly increased, and the activities of SOD and GSH were significantly decreased (P<0. 0). 2The edema in each group under light microscope was the heaviest in the injury group, followed by the treatment group and the lightest in the control group. There was central hemorrhage in the injury group and the treatment group, and the injury group was heavier; the neuron vacuoles appeared in the injury group at 3 hours. Denaturation, karyolysis or pyknosis, Nissl bodies disappeared; 6h partial nerve fiber demyelination or rupture occurred. The treatment group had different degrees of recovery. Conclusion Ginsenoside has a protective effect on early spinal cord injury.