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目的了解研究母鼠妊娠期和哺乳期沥青烟染毒对仔鼠学习记忆和海马组织脏器系数的影响。方法昆明种成年母鼠40只,随机分成高、中、低剂量组和对照组,每组10只。母鼠自孕第1 d置于1 m3静式染毒柜内,开始沥青烟染毒2 h/d,至仔鼠出生后21 d断乳。染毒剂量为165(LD0)、82(1/2 LD0)、41(1/4 LD0)mg/m3。分别于出生后第7、14、21 d测定仔鼠体重和海马组织重量并计算其脏器系数;21 d用Y型迷宫测定仔鼠学习记忆能力T1、T2、T3(以仔鼠连续10次测试中成功9次即为获得记忆,以总测试次数减10得到的差值记为T1,3 d后再用同样方法复测,结果记为T2,以公式(T1-T2)/T1×100%计算记忆保持率记为T3。测定或检查时每窝随机取1只仔鼠,每组取10只。结果高剂量染毒组仔鼠21 d体重显著低于对照组(P<0.05);出生不同时间点高剂量染毒组仔鼠海马组织重量及其脏器系数显著高于对照组(P<0.01);Y型迷宫实验结果显示,仔鼠出生后各时间点中、高剂量组T1、T2高于对照组,T3低于对照组,差异均有统计学意义(P<0.001)。结论妊娠期和哺乳期母体高剂量沥青烟染毒可减缓仔鼠体重的正常增长;同时可影响仔鼠海马组织的正常发育并降低其学习记忆能力。
Objective To study the effects of asparagus poisoning during pregnancy and lactation on the learning and memory and the organ coefficient of hippocampus in offspring. Methods Forty Kunming adult female rats were randomly divided into high, medium and low dose groups and control group, with 10 in each group. The female rats were placed in 1 m3 static-type poisoning cabinet on the first day of their pregnancy, and began to smoke the asphalt for 2 h / d, till the weaned mice became weaned 21 days after their birth. Exposure doses were 165 (LD0), 82 (1/2 LD0), 41 (1/4 LD0) mg / m3. The body weight and the weight of hippocampus were measured on the 7th, 14th and 21st day after birth, and the organ coefficients were calculated. On the 21st day, the learning and memory abilities of the pups T1, T2 and T3 were determined by Y-maze test The test was successful nine times to obtain memory, the total number of tests minus 10 get the difference recorded as T1,3 d and then retest by the same method, the result is recorded as T2, the formula (T1-T2) / T1 × 100 % Was calculated as the retention rate of T3, and one offspring was randomly taken from each litter at the time of determination or examination.Results The body weight of the offspring of high dose exposure group was significantly lower than that of the control group on the 21st day (P <0.05); The weight and organ coefficient of hippocampus of pups exposed to high dose at different time points were significantly higher than those of control group (P <0.01). The results of Y-maze test showed that at each time point after birth, , T2 was higher than that of the control group, and T3 was lower than that of the control group (P <0.001) .Conclusion Pregnant and lactating maternal high-dose bitumen smoke could retard the normal growth of the weight of the offspring, The normal development of hippocampal tissues of pups and their learning and memory abilities.