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目的探讨Epstein-Barr病毒(EBV)感染在系统性红斑狼疮(SLE)病因学中的作用以及EBV活动性感染与疾病活动期的关系。方法PCR技术检测85例SLE患者和43例正常人外周血单一核细胞中EBVDNA,采用巢式PCR(Nested-PCR)技术检测EBVDNA阳性标本EBV增殖期基因BZLF1mRNA的表达,ELISA法检测血清EBV特异性IgM抗体。结果SLE患者组EBVDNA阳性率明显高于对照组(P<0.05),EBV即刻早期基因BZLF1mRNA的表达在SLE患者组与对照组中阳性率差异无显著性(P>0.05),ELISA法检测血清EBV特异性IgM抗体,SLE患者组阳性率高于对照组(P<0.05)。结论SLE患者EBVDNA阳性率高于正常对照组,部分SLE患者存在EBV活动性感染,EBV感染可能与SLE的发生发展有关。
Objective To investigate the role of Epstein-Barr virus (EBV) infection in the etiology of systemic lupus erythematosus (SLE) and the relationship between active EBV infection and disease activity. Methods EBV DNA was detected by PCR in peripheral blood mononuclear cells of 85 SLE patients and 43 normal controls. The expression of BZLF1 mRNA in EBV proliferative phase was detected by nested PCR (Nested-PCR), and the serum EBV specificity IgM antibody. Results The positive rate of EBV DNA in patients with SLE was significantly higher than that of the control (P <0.05). There was no significant difference in the expression of BZLF1 mRNA between the patients with SLE and controls (P> 0.05) The positive rate of specific IgM antibody and SLE patients was higher than that of the control group (P <0.05). Conclusions The positive rate of EBV DNA in patients with SLE is higher than that of normal controls. Some patients with SLE have active EBV infection. EBV infection may be related to the development of SLE.