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The paper analyzes the radon data of nearly two decades on the Jiayuguan fault zone, discusses the main influencing factors, and puts forward the relationship between radon and air temperature, ground temperature and rainfall. We summarized the earthquake reflecting effect for ML≥5. 0 about 400km within the Jiayuguan station, and reached the conclusion that it has better earthquake-reflecting ability before an earthquake, usually appearing as abnormal changes in sustained low value. By extracting the annual trend of radon in Jiayuguan station over many years, we discovered that the annual trend of radon has a close relationship with the seismic activity in surrounding areas, namely, if the annual variation of radon is larger, the seismic activity in surrounding areas is stronger; Otherwise, if the annual variation of radon is relatively stable, the seismic activity in the vicinity is weak.