论文部分内容阅读
目的评价~(125)I粒子植入预防犬良性尿道狭窄内切开术后复发疗效,并观察犬尿道瘢痕组织中转化因子β_1(TGFβ_1)的表达。方法选取16只健康雄性成年犬,采用小儿电切镜制作犬尿道狭窄动物模型,待模型制作成功,全身麻醉后行尿道狭窄切开术。术后5 d因感染死亡1只犬。将成年犬随机分为~(125)I粒子未置入粒子组(7只)和置入粒子组(8只)。术后4周,检查膀胱镜观察尿道情况。同时,应用免疫组织化学方法检测犬尿道狭窄段瘢痕组织中TGFβ_1的表达,对比两组犬动物模型的TGFβ_1表达情况。结果置入粒子组形成尿道狭窄的1例(12.5%),未置入粒子组形成尿道狭窄的7例(100.0%),比较差异有统计学意义(P<0.05)。置入粒子组尿道瘢痕组织的TGFβ_1表达较未置入粒子组少,差异有统计学意义(P<0.05)。结论 ~(125)I粒子植入对预防尿道狭窄有重要作用,其抑制了TGFβ_1表达,进而抑制瘢痕形成,这为临床研究提供了动物实验的实验依据,对于其他相关的临床研究具有一定的指导意义。
Objective To evaluate the effect of ~ (125) I seed implantation in preventing the recurrence of benign urethral stricture in dogs and observe the expression of transforming growth factor β 1 (TGFβ_1) in the kyphus scar tissue. Methods Sixteen healthy male adult dogs were selected and pediatric urethral stricture model was made by pediatric resectoscope. When the model was successfully made, urethral stricture incision was performed after general anesthesia. Five dogs died of infection after 5 days. Adult dogs were randomly divided into ~ (125) I particles not placed in the particle group (7) and placed in the particle group (8). Four weeks after operation, cystoscopy was performed to observe the urethra. At the same time, immunohistochemistry was used to detect the expression of TGFβ_1 in the scar tissue of dog urethral stricture. The expression of TGFβ 1 in two groups of dogs was compared. Results One case (12.5%) of urethral stricture was placed in the group of particles and 7 cases (100.0%) of the urethral stricture were not placed in the group of particles, the difference was statistically significant (P <0.05). The expression of TGFβ_1 in the urethral scar tissue was smaller than that in the untreated group, the difference was statistically significant (P <0.05). Conclusion The 125 I seed implantation plays an important role in the prevention of urethral stricture, which inhibits the expression of TGFβ_1 and then inhibits the formation of scars. This provides the experimental basis for clinical research and has some guidance for other related clinical studies significance.