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目的：研究脑组织细胞膜的制备和鉴定方法。方法：采用匀浆及蔗糖密度梯度离心法制备脑组织细胞膜，通过质膜标志酶活性测定及电镜观察对所得之膜制品进行鉴定。结果；经密度梯度离心后的膜制品具较高的5’-核苷酸酶活性，电镜照片显示清晰的膜相结构。结论：该方法的制备物为较为纯化的脑组织细胞质膜。
Objective: To study the preparation and identification of brain cell membrane. Methods: The plasma membrane of brain tissue was prepared by homogenization and sucrose density gradient centrifugation. The membrane-derived products were identified by measuring the activity of plasma membrane-labeled enzymes and electron microscopy. Results: The membrane products after density gradient centrifugation had higher 5’-nucleotidase activity and the electron microscope images showed a clear membrane phase structure. Conclusion: The preparation of this method is a more purified cytoplasmic membrane of brain tissue.