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本文对国内外研究者设计的11对PCR引物检测柑桔黄龙病菌的特异性进行了比较。其中包括5对一步法PCR引物(A2/J5、GB1/GB3、MP1/MP2、CQULA03F/CQULA03R和CLA-F2/CLA-R2)和6对巢式PCR引物(P1/P2、P3/P4、LP1/LP2、LP3/LP4、DP1/DP2和DP3/DP4)。结果发现,MP1/MP2和CLA-F2/CLA-R2 2对引物具有非特异性,不能区分亚洲柑桔黄龙病菌和非洲柑桔黄龙病菌,其余的9对引物特异性较好。本文旨在为研究者选择柑桔黄龙病菌扩增引物提供参考。
In this paper, 11 pairs of PCR primers designed by researchers at home and abroad were compared for their specificity. Including 5 pairs of one-step PCR primers (A2 / J5, GB1 / GB3, MP1 / MP2, CQULA03F / CQULA03R and CLA-F2 / CLA-R2) and 6 pairs of nested PCR primers (P1 / P2, P3 / P4, LP1 / LP2, LP3 / LP4, DP1 / DP2 and DP3 / DP4). The results showed that MP1 / MP2 and CLA-F2 / CLA-R2 2 were non-specific to the primers, could not distinguish between the Asian Citrus Huanglongbing and the African Citrus Huanglongsai, and the other 9 pairs of primers had good specificity. The purpose of this article is to provide a reference for the researchers to choose the primers for amplifying citrus yellow dragonfly.