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利用赤霉菌毒素作选择压,在MS基上用小麦品种间杂交幼胚作外植体,研究了它们愈伤组织形成及幼苗分化;分化成株的再生植株在田间接种鉴定了它们的抗病性。在毒素作用下.品种间杂交幼胚外植体可形成愈伤组织并分化成苗,但数量明显低于未经毒素处理的外植体。田间抗性鉴定结果,不论是中抗组合,还是抗病组合中.毒素压下获得的再生株的抗性比亲本都有增强,中抗组合中高抗单株出现率平均比亲本增加35.7%,抗病组合中抗病单株比亲本增加32%。差异显著性测定结果.利用品种间杂交幼胚作外植体离体筛选抗病再生株,亲本抗性很关键,亲本抗性强.其再生株的抗性提高的幅度大.筛选出抗病单株的机率就越大。
Using Gibberella toxin as selective pressure, the embryos were used as explants on the basis of MS hybridization to study their callus formation and seedling differentiation. The regenerated plants that differentiated into plants were inoculated in the field to identify their resistance Sex. Under the action of toxins. Intercropping of embryos with immature embryos could form callus and differentiate into seedlings, but the number was significantly lower than that without explant. Field resistance identification results, whether in the anti-combination, or disease-resistant combination. The resistance of the regenerated plants obtained from the toxin depressing was stronger than that of the parents, and the occurrence rate of high resistant plants in the medium resistant combinations was 35.7% higher than that of the parents and that of the resistant ones in the resistant combinations was 32% higher than that of the parents. Difference significance test results. In vitro selection of resistant regenerated plants by crossing embryos of immature embryos with hybrid embryos, the resistance of the parents is very important, and the parents are highly resistant. The resistance of the regenerated strain is greatly increased. The more likely it is to screen out resistant plants.