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目的:探讨糖尿病肾病(diabetes kidney disease, DKD)模型小鼠肠肾组织晚期糖基化终产物(advanced glycosylation end product, AGEs)/钠-葡萄糖1型协同转运体(sodium-glucose cotransporter-1, SGLT-1)及肠道菌群的变化。方法:选取KKay小鼠20只，分为糖尿病组(DM组，n n＝10)和糖尿病肾病组(DKD组，n n＝10)，检测血清AGEs、脂多糖(lipopolysaccharide, LPS)、肿瘤坏死因子α(tumor necrosis factor-α，TNF-α)、白细胞介素6(intereukin-6，IL-6)的浓度，Western blot技术检测AGEs及SGLT-1在肠肾组织的蛋白表达，高通量测序分析肠道菌群的差异。n 结果:与DM组比较，DKD组小鼠炎症指标TNF-α、IL-6及内毒素水平升高，血清、肠肾组织中AGEs的含量升高、SGLT-1在肠道组织含量升高(n P<0.05)。Metastats检验显示DKD组门水平上疣状微生物菌门丰度减低，变形菌门丰度升高(n P<0.05)，气单胞菌属、肠杆菌属、摩根氏菌属、克雷伯菌属、沙雷氏菌属、伯克霍尔德菌属等Gn -菌相对优势分布，阿克曼氏菌属丰度显著低于DM组(n P<0.05)。n 结论:DKD小鼠肠道AGEs的升高可能诱发肠道菌群失调，尤其是变形菌门升高、疣状微生物菌门及阿克曼氏菌属降低，导致Gn -菌渗漏入血产生肠源性内毒素血症引起炎症反应，这可能是DKD发病的重要因素。SGLT-1在肠道组织升高，这可能进一步参与DKD的病情发展。n “,”Objective:To investigate the changes of advanced glycosylation end product(AGEs)/sodium-glucose cotransporter-1(SGLT-1) in intestinal and renal tissues and intestinal flora of mice with diabetes kidney disease.Methods:Twenty KKay mice were divided into diabetic group(DM group, n n=10) and diabetic kidney disease group(DKD group, n n=10). The concentrations of serum AGEs, lipopolysaccharide(LPS), tumor necrosis factor-α(TNF-α), and intereukin-6(IL-6) were measured. Western blot technique was used to detect the protein expression of AGEs and SGLT-1 in kidney and intestinal tissue, and high-throughput sequencing was used to analyze the difference of intestinal flora.n Results:The levels of inflammatory markers TNF-α, IL-6, and serum endotoxin in DKD group were significantly higher than those in DM group(n P<0.05). The contents of AGEs in serum and intestine and kidney were increased, and the contents of SGLT-1 in intestine and kidney were increased(n P<0.05). Metastats test showed that the abundance of Verrucomicrobia decreased and the abundance of Proteobacteria increased in DKD group(n P<0.05). Gn - bacteria such as Aeromonas, Enterobacter, Morgan, Klebsiella, Serratia, and Burkholderia were relatively dominant, and the abundance of Akkermansia was significantly lower than that in DM group(n P<0.05).n Conclusion:The increase of AGEs in intestinal tract of DKD mice may induce intestinal dysbacteriosis, especially the increase of Proteobacteria, the decrease of Verrucosa and Wilhelm Ackermann, and the leakage of G-bacteria into the blood to produce intestinal endotoxemia and cause inflammatory reaction, this may be an important factor in the development of DKD. SGLT-1 is elevated in intestinal tissue, which may be involved in the development of DKD.