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以甜瓜品种‘羊角酥瓜’为试材,利用人工气候室控制环境条件(昼/夜25/18℃),研究盐胁迫条件下外源褪黑素(MT)和Ca~(2+)对甜瓜幼苗根系和叶片中Cl~-、Na~+、K~+、Mg~(2+)、Ca~(2+)离子含量,Na~+/K~+、Na~+/Ca~(2+)、Na~+/Mg~(2+)值,以及H~+-ATP酶活性、渗透调节物质积累和细胞膜质过氧化的影响.结果表明:与对照相比,盐胁迫处理显著抑制甜瓜幼苗生长,增加根系和叶片中Cl-、Na~+含量,降低K~+、Mg~(2+)、Ca~(2+)含量.盐胁迫下,喷施外源MT或Ca~(2+)处理均可以显著降低甜瓜根系和叶片中Cl~-、Na~+含量,提高K~+、Mg~(2+)、Ca~(2+)含量,植株体内Na~+/K~+、Na~+/Ca~(2+)和Na~+/Mg~(2+)值下降;同时也提高了根系和叶片H~+-ATP酶活性及叶片渗透调节物质的含量,降低盐胁迫对细胞膜的伤害,表现在甜瓜叶片相对电导率和丙二醛含量降低.总之,在盐胁迫条件下,外源MT、Ca~(2+)单独和复配处理均可通过提高H~+-ATP酶活性来降低盐害离子的含量,改善甜瓜幼苗中的离子平衡,同时增加渗透调节物质的含量,降低膜质过氧化水平,从而增强其对盐胁迫的适应性,其中MT和Ca~(2+)复配处理时的效果更好.复配外施MT和Ca~(2+)在诱导甜瓜幼苗提高耐盐方面具有协同增效作用.
The melon variety ’Crouching melon’ was used as material to control the effects of exogenous melatonin (MT) and Ca ~ (2+) under salt stress conditions under controlled environmental conditions (day / night 25/18 ℃) The content of Cl ~ -, Na ~ +, K ~ +, Mg ~ (2 +) and Ca ~ (2+) in the roots and leaves of muskmelon seedlings were increased with the increase of Na ~ + / K ~ + and Na ~ + / Ca ~ +, Na ~ + / Mg ~ (2+) and H ~ + -ATPase activity, osmotic adjustment substance accumulation and cell membrane peroxidation.The results showed that salt stress significantly inhibited the growth of melon The content of Cl ~, Na ~ + in roots and leaves and the content of K ~ +, Mg ~ (2 +) and Ca ~ (2+) in seedlings increased under salt stress.Exogenous MT or Ca ~ (2) +) Could significantly decrease the content of Cl ~ -, Na ~ + in the roots and leaves of muskmelon, increase the contents of K ~ +, Mg ~ (2 +) and Ca ~ , Na ~ + / Ca ~ (2+) and Na ~ + / Mg ~ (2+) decreased. At the same time, H ~ + -ATPase activity and leaf osmotic adjustment substance content in roots and leaves were also increased, The damage of cell membrane showed that the relative electric conductivity and malondialdehyde content of melon leaves were decreased.In short, under the condition of salt stress, exogenous MT and Ca ~ (2+) alone and compound treatment could increase H ~ + - ATP enzyme activity To reduce salt ion content, improve ion balance in melon seedlings, increase the content of osmotic adjustment substances and reduce the level of membrane lipid peroxidation, thereby enhancing their adaptability to salt stress. Among them, MT and Ca ~ (2+) The effect of compounding treatment is better.The compound application of MT and Ca ~ (2+) have synergistic effect in inducing melon seedlings to improve salt tolerance.