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目的分析2011-2014年河南地区5岁以下婴幼儿病毒性腹泻感染状况、季节流行特征及轮状病毒(HRV)血清型基因分型。方法收集2011年12月-2014年12月单中心郑州市儿童医院住院腹泻患儿粪便共506份。由中国疾病预防控制中心检测轮状病毒(HRV),人星状病毒(HAst V)、人杯状病毒(Hu CV)、肠道腺病毒(EAd V)及轮状病毒阳性标本血清型基因分型检测。结果检测的506份标本中HRV(224)44.26%、HAst V(83)16.40%、Hu CV(诺如病毒II型为主)(58)11.46%、EAd V(32)6.32%、未定型(109)21.54%,HRV是导致河南地区婴幼儿病毒性腹泻的主要病原;每年秋冬季节患儿病毒阳性率检出明显升高;病毒性腹泻各组间所占比例在男女性别上无显著差异;腹泻患儿发病的高峰年龄为6~24月龄,占51.54%(183/355);224份RV检测阳性标本G血清型和P基因型分布特征,G血清型流行优势毒株为G3型90份(40.17%),未发现G8型。P基因型分型结果显示,最常见的毒株是P8型99份(44.19%)。基因型组合:以G3P[8]为主;506份检测标本中混合感染共24份(4.74%),以轮状病毒混合其他病毒感染为主,占50%(12/24),轮状病毒和人星状病毒混合感染最多,占29.16%(7/24);其次,轮状病毒和人杯状病毒混合感染占20.83%(5/24)。混合感染的患儿中<2岁的占83.33%(20/24)。结论河南地区5岁以下儿童病毒性腹泻的病原分型复杂,以轮状病毒检出率高,发病高峰在每年秋冬季节,以6~24月龄婴幼儿为主,HRV主要亚型G3[P8],同时存在不同病原混合感染的现象,具有明显的季节差异和人群分布。
Objective To analyze the prevalence of infectious diarrhea, seasonal epidemics and genotyping of rotavirus (HRV) serotypes in infants under 5 years of age in Henan province during 2011-2014. Methods A total of 506 children with diarrhea in Zhengzhou Children’s Hospital from December 2011 to December 2014 were collected. The serotypes of rotavirus (HRV), human astrovirus (HAst V), Hu CV, intestinal adenovirus (EAd V) and rotavirus positive samples were detected by Chinese Center for Disease Control and Prevention Type testing. Results Forty-six specimens of HRV (224), 44.26%, HAst V (83) 16.40%, Hu CV (Norovirus type II) (58) 11.46%, EAd V (32) 6.32% 109) 21.54%, HRV is the leading cause of infant viral diarrhea in Henan Province; the positive rate of virus in children in autumn and winter seasons was significantly higher; the proportion of viral diarrhea among the groups was no significant difference between men and women; The onset age of children with diarrhea was 6-24 months old, accounting for 51.54% (183/355). The distribution of serogroups and P genotypes in 224 RV positive specimens was G type 90 (40.17%), did not find G8 type. P genotyping results showed that the most common strains were 99 P8 (44.19%). The genotypes consisted of G3P [8]. A total of 24 samples (4.74%) were found in 506 samples. The majority were rotavirus mixed with other viruses, accounting for 50% (12/24), rotavirus And human astrovirus, which accounted for 29.16% (7/24), followed by mixed infection with rotavirus and human calicivirus (20.83%, 5/24). Children <2 years of mixed infection accounted for 83.33% (20/24). Conclusions The pathogen classification of viral diarrhea in children under 5 years of age in Henan is complicated. The detection rate of rotavirus is high, and the incidence peak is in autumn and winter every year. The majority of infants are 6-24 months old. The major subtype of GV [P8 ], While there is a mixed infection of different pathogens, with significant seasonal differences and population distribution.