论文部分内容阅读
目的:比较旋毛虫成虫抗原和肌幼虫抗原诱导小鼠产生保护性免疫效果的异同。方法:制备旋毛虫成虫抗原和肌幼虫抗原,分组免疫小鼠,并于末次免疫后10d用旋毛虫感染性幼虫对各组小鼠实施攻击感染,检测攻击感染后各组小鼠肠道成虫和肌肉幼虫数并计算减虫率,同时采用ELISA法检测各组小鼠血清IgG抗体水平。结果:成虫抗原免疫组小鼠和肌幼虫抗原免疫组小鼠检获的肠道成虫数和肌幼虫数均显著少于对照组(P<0.05),其中以成虫抗原免疫组小鼠减虫率最高(P<0.01)。成虫抗原免疫组和肌幼虫抗原免疫组小鼠于实施旋毛虫感染性幼虫进行攻击感染的当日所检测的血清IgG抗体水平与其免疫前相比均升高(P<0.01,P<0.05),与对照组相比亦升高(P<0.01,P<0.05),其中也以成虫抗原免疫组小鼠血清IgG抗体升高最为显著(P<0.01)。结论:旋毛虫成虫抗原和肌幼虫抗原均能激发小鼠产生保护性免疫,其中成虫抗原免疫组小鼠显示出更好的抗感染保护性。
OBJECTIVE: To compare the similarities and differences of protective immunity induced by adult Trichinella spiralis antigen and muscle larval antigen. Methods: Adult Trichinella spiralis antigens and muscle larvae antigens were prepared and immunized mice were divided into groups. At the 10th day after the last immunization, the infected mice were challenged with Trichinella spiralis larvae, and the intestinal motility and The number of muscle larvae was calculated and the worm reduction rate was calculated. At the same time, the level of serum IgG was detected by ELISA. Results: The numbers of adult worms and muscle larvae in the immunized mice and the muscle larvae antigen immunized mice were significantly less than those in the control group (P <0.05). The worm resistance rate in the immunized mice was the highest (P <0.01). The level of serum IgG antibodies detected on the day of challenge infection with Trichinella spiralis larvae was higher than that before immunization (P <0.01, P <0.05), and (P <0.01, P <0.05). Serum IgG antibody of the immunized mice also showed the most significant increase (P <0.01). CONCLUSION: Trichinella spiralis adult antigen and muscular larval antigen can both induce protective immunity in mice. Among them, adult mouse antigen immunized mice show better protection against infection.