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目的探讨原花青素(Oligomeric proanthocyanidin,OPC)干预对高脂饮食小鼠脂质代谢的影响。方法雄性C57BL/6小鼠48只按体重随机分为4组:A:对照组(Control);B:Con+OPC组;C:高脂组(High fat,HF);D:HF+OPC组,其中B和D组给予OPC(100 mg/kg/d)干预10周。测定血清总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG),丙氨酸氨基转氨酶(Alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(Aspartate aminotransferase,AST)以及肝脏TC和TG的水平,通过实时定量PCR法检测肝脏脂质代谢关键基因mRNA的表达水平。结果 HF组小鼠血清TG、TC、ALT以及肝脏TG的含量显著高于对照组(P<0.05);OPC干预显著降低HF组小鼠血清TC水平、ALT活性以及肝脏TC和TG含量(P<0.05),提高HF组小鼠肝脏三磷酸腺苷结合盒转运体A1(ATP-binding cassette transporter-1,ABCA1)mRNA的表达水平(P<0.05)。结论 OPC干预能够有效改善高脂饮食小鼠血清和肝脏脂质代谢紊乱的情况。
Objective To investigate the effect of OPC on the lipid metabolism in high fat diet mice. Methods 48 male C57BL / 6 mice were randomly divided into 4 groups according to body weight: A: Control group; B: Con + OPC group; C: High fat group; , In which B and D groups were given OPC (100 mg / kg / d) for 10 weeks. Total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver TC and TG The level of mRNA expression of key genes in liver lipid metabolism was detected by real-time quantitative PCR. Results Serum levels of TG, TC, ALT and liver TG in HF group were significantly higher than those in control group (P <0.05). The levels of TC, ALT and TC and TG in hepatic tissue of HF group were decreased significantly (P < 0.05), and increased the hepatic ATP-binding cassette transporter-1 (ABCA1) mRNA expression in HF group (P <0.05). Conclusion OPC intervention can effectively improve the lipid metabolism disorders of serum and liver in high-fat diet mice.