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【目的】研究盐胁迫对番茄幼苗抗氧化酶活性和同工酶的影响。【方法】以番茄(Lycopersicon esculentum L.)F144种子为材料,种子萌发2d后用120mmol/L NaCl处理7d,测定幼苗谷胱甘肽还原酶(GR),谷胱甘肽过氧化物酶(GPX),过氧化氢酶(CAT),超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)的活性以及同工酶。【结果】在120mmol/L NaCl作用下,CAT和APX活性显著提高,GR和GPX活性提高不显著,而SOD活性无显著变化。SDS-PAGE分析发现,番茄幼苗叶中的GR有两条同工酶,分子量分别为50kDa和46kDa,GPX有3条同工酶,分子量分别为60、50和48kDa,CAT有两条主要的同工酶,APX有3条主要同工酶,SOD有3条同工酶;在120mmol/L NaCl作用下,GR中分子量为46kDa的同工酶消失,GPX中的48kDa和60kDa同工酶受到显著抑制,CATⅠ、CATⅡ和APXⅡ增强,而SOD的同工酶没有变化。【结论】CAT和APX是主要的盐胁迫抗氧化清除剂。
【Objective】 To study the effects of salt stress on antioxidant enzyme activities and isozymes in tomato seedlings. 【Method】 The seeds of tomato (Lycopersicon esculentum L.) F144 were germinated for 2 days and then treated with 120 mmol / L NaCl for 7 days. The contents of glutathione reductase (GR), glutathione peroxidase (GPX) ), Catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) activity and isozymes. 【Result】 The activities of CAT and APX were significantly increased under the action of 120 mmol / L NaCl, the activities of GR and GPX were not significantly increased, while the activities of SOD did not change significantly. SDS-PAGE analysis showed that there are two isozymes in GR of tomato seedling leaves with molecular weights of 50kDa and 46kDa, three isozymes of GPX, molecular weights of 60, 50 and 48kDa respectively, CAT has two major There were 3 major isoenzymes in APX and 3 isoenzymes in SOD. The isoenzyme of 46kDa in GR disappeared under the action of 120mmol / L NaCl, and the 48kDa and 60kDa isozymes in GPX were significantly affected Inhibition, CAT Ⅰ, CAT Ⅱ and APX Ⅱ enhanced, while the SOD isoenzyme did not change. 【Conclusion】 CAT and APX are the main salt stress antioxidant scavengers.