【摘 要】
：
目的确定在一个质粒载体上串联目的操纵子以提高目的蛋白表达量的可行性,阐明宿主细胞对胞内质粒DNA总量调控的可能机制.方法亚克隆构建了两组操纵子正向串联的表达质粒:CWll
【基金项目】
：
国家高技术研究发展计划(863计划);
论文部分内容阅读
目的确定在一个质粒载体上串联目的操纵子以提高目的蛋白表达量的可行性,阐明宿主细胞对胞内质粒DNA总量调控的可能机制.方法亚克隆构建了两组操纵子正向串联的表达质粒:CWll系列分别含1-4个正向操纵子,质粒大小以2.25 kb的增加量从5.47 kb增加至12.26 kb;CW12系列分别含1-3个正向操纵子,质粒大小以2.16 kb的增加量从5.40 kb增加至9.72 kb.SDS凝胶电泳和激光密度扫描测定目的蛋白表达量;3H-TdR掺入法测定质粒拷贝数.结果操纵子的串联不影响宿主大肠杆菌的生长;温度诱导表达后CW11系列目的蛋白表达量分别为菌体总蛋白的44.9%±3.9%、51.3%±4.1%、54.8%±3.3%和58.2%±3.4%,CW12系列目的蛋白表达量分别为菌体总蛋白的32.2%±5.0%、42.8%±4.1%和46.9%±4.0%.两组质粒的拷贝数均随操纵子串联个数的增加而显著减少(P
其他文献
Transforming growth factorβ1 (TGFβ1) is known to be intimately involved in many cellular processes. To explore the mechanism of TGFβ1 in these processes, the
Objective: To examine the protective effect of Ginkgo biloba leaf extract (GbE) on learning and memory deficit induced by aluminum chloride (AlCl3), and explore
随着经济的推移,我国经济的发展,以及网络的广泛普及,我国各行业的发展速度均有了明显的提升。医院做为保障人类生命健康的重要部门,其计算机网络信息化建设有着极其重要的意
目的探讨晚期肝癌介入治疗综合措施,进一步提高临床疗效.方法对33例肝癌介入治疗晚期综合症状采取一系列治疗措施.21例行经皮股动脉药盒导管系统(PCS)植入术;出血应用凝血酶
In order to establish more simple and effective rat orthotopic lung transplantation models, 20 rats were divided into donor and recipient groups. Rat lung trans
In our previous study, we proposed that there may be an unknown pathway in the upper stream of the known signal transduction pathway of Ciliary neurotrophic fac
Flounder gill (FG) cells were used to isolate lymphocystis disease virus (LCDV) and two monoclonal antibodies (Mabs) (1A8 and 3G3) against LCDV were used to tra
The relationship between hypermethylation of CpG islands in the promoter regions of O6methylguanine DNA methyltransferase (MGMT)genes and laryngeal squamous cel
Objective To study the oncogenic potential of mouse translation initiation factor 3 (TIF3) and elongation factor-1δ (TEF-1δ) in malignant transformed human br
目的肾上腺髓质素(AM)是一种具有血管舒张活性的多肽物质,可降低肺动脉高压.本研究通过观察慢性低氧大鼠肺内AM及其受体(AMR)的表达情况,探讨AM在低氧性肺动脉高压发病中的作