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研究添加外源褪黑素对水稻幼芽Ni胁迫的缓解作用.结果表明,较低浓度Ni(10μmol·L~(-1)、50μmol·L~(-1))胁迫对水稻幼芽根系生长影响不大,而较高浓度Ni(100~1 000μmol·L~(-1))胁迫显著地抑制水稻根系的生长.与CK处理相比,100~1 000μmol·L~(-1)Ni胁迫导致水稻总根长降低63.3%~98.0%,根表面积降低56.9%~96.3%.褪黑素的添加能明显地促进Ni胁迫下水稻幼芽的生长,尤其是对幼芽的根系生长促进作用更明显.当Ni胁迫浓度为100μmol·L~(-1)时,两个水稻品种总根长相较于无Ni胁迫的对照组降低了58.4%~83.8%,在添加10μmol·L~(-1)褪黑素处理后,水稻总根长相较于无Ni胁迫的对照组仅降低了8.7%~29.1%.添加外源褪黑素导致Ni胁迫下水稻幼芽的O_2~(-·)产生速率和相对电导率显著降低,CAT活性和可溶性蛋白含量显著提高.与100μmol·L~(-1)Ni胁迫处理相比,添加10μmol·L~(-1)褪黑素能显著地降低两种水稻品种O_2~(-·)产生速率43.2%~50.2%、相对电导率25.7%~31.6%,提高CAT活性21.9%~33.7%、可溶性蛋白含量82.6%~84.6%.褪黑素可以有效地缓解Ni胁迫对水稻幼芽的毒害作用.
The effects of exogenous melatonin supplementation on the allelopathic effects of Ni on the shoots of rice seedlings were studied.The results showed that the root growth of the shoots was inhibited by lower concentrations of Ni (10μmol·L -1, 50μmol·L -1) (100-1000 μmol·L -1) Ni significantly inhibited the growth of rice roots.Compared with the CK treatment, 100-1000 μmol·L -1 Ni stress Resulting in a decrease of total root length of 63.3% -98.0% and a decrease of root surface area of 56.9% -96.3% .The addition of melatonin could obviously promote the growth of young shoots under the stress of Ni, especially on the root growth of young shoots The total root length of two rice cultivars decreased by 58.4% -83.8% compared with the control without Ni stress when the Ni stress concentration was 100 μmol·L -1, Melatonin treatment decreased the total root length of rice by only 8.7% -29.1% compared with the control without Ni stress. The addition of exogenous melatonin resulted in the increase of O 2 - (- ·) production rate of rice seedlings under Ni stress and The relative electrical conductivity decreased significantly and CAT activity and soluble protein content increased significantly.Compared with 100μmol·L -1 Ni stress, adding 10μmol·L -1 melatonin significantly reduced the content of CAT and soluble protein O_2 ~ (- ·) The results showed that melatonin could effectively alleviate the toxicity of Ni stress to the sprouts of rice by increasing the rate of bioaccumulation by 43.2% -50.2%, the relative conductivity by 25.7% -31.6%, the CAT activity by 21.9% -33.7% and the soluble protein content by 82.6% -84.6% effect.