论文部分内容阅读
[目的]解决采用抗生素抑菌导致转基因马铃薯植株生长与分化受到抑制的问题。[方法]利用转基因植株诱导试管微型薯,由试管微型薯抽枝获得转基因脱菌植株。[结果]参试3个马铃薯品种中,SⅠ、SⅡ最佳试管微型薯诱导培养基为MS+6-BA0.5mg/L+GA30.1mg/L+cef150mg/L,NT最佳试管微型薯诱导培养基为MS+ZT0.5mg/L+GA30.1mg/L+cef150mg/L;薯块抽枝最佳培养基为MS+ZT0.5mg/L+NAA0.1mg/L;薯块直径0.5-0.7cm抽枝数最高;转基因脱菌植株在无抗生素快繁培养基中经30d培养污染率为0,脱菌植株茎段粗壮,无分枝现象。[结论]建立的方法脱菌简单易行,为转基因个体的筛选和生长排除了障碍。
[Objective] The research aimed to solve the problem of inhibition of growth and differentiation of transgenic potato plants by antibiotic antibacterial. [Method] Transgenic plants were used to induce microtubes in vitro. [Result] Among the three potato cultivars, the best S Ⅰ and SⅡ microtuber-inducing medium in vitro was MS + 6-BA0.5mg / L + GA30.1mg / L + Cef150mg / L, Medium for MS + ZT0.5mg / L + GA30.1mg / L + cef150mg / L; potato block the best medium for the MS + ZT0.5mg / L + NAA0.1mg / L; cm. The highest contamination rate was observed in the untreated transgenic plants. The contamination rate of the transgenic plants was 0 in the medium without antibiotics and the stalks of the sterilized plants were stout with no branching. [Conclusion] The established method was simple and easy to remove bacteria, which obstructed the screening and growth of transgenic individuals.