论文部分内容阅读
异戊烯焦磷酸异构酶(IPI)是萜类合成途径的关键酶之一。本文在丹参转录组高通量数据分析的基础上,对丹参IPI基因(SmIPI)进行了克隆及序列分析。SmIPI全长1234bp,包含681bp的开放读码框,编码226个氨基酸。生物信息学结构分析表明,SmIPI为亲水性α/β蛋白,包含有IPI结构域,在序列组成、结构及活性位点等方面与其他植物的IPI均具有高度的相似性。实时荧光定量PCR分析结果表明,SmIPI在丹参生长的各个时期和不同组织器官中差异表达,其表达受病原菌和茉莉酸甲酯的诱导。
Isopentene pyrophosphate isomerase (IPI) is one of the key enzymes in the terpenoid biosynthesis pathway. Based on the high-throughput data analysis of Salvia miltiorrhiza transcriptome, we cloned and sequenced the SmIPI gene of Salvia miltiorrhiza. SmIPI full-length 1234bp, contains 681bp open reading frame, encoding 226 amino acids. Bioinformatics structural analysis showed that SmIPI is a hydrophilic α / β protein and contains IPI domain, which has high similarity with other plant IPIs in terms of sequence composition, structure and active sites. Real-time PCR analysis showed that SmIPI was differentially expressed in different tissues and organs during the growth of Salvia miltiorrhiza, and its expression was induced by pathogen and methyl jasmonate.