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目的采用毛细管电泳法研究并建立葛根药材的指纹图谱。方法微乳液电动毛细管色谱法。未涂层熔融石英毛细管(63.5cm×50.0cm,50.0μm),缓冲液为微乳液,电压20kV,检测波长254nm。结果以葛根素峰为参照峰,建立了广西葛根药材的微乳液电动毛细管色谱指纹图谱,确认了13个共有特征指纹峰,经加样法标识的成分是大豆苷、葛根素、大豆苷元和染料木素。评价了3个不同产地和4种不同提取方法所得葛根指纹图谱的相似度均在0.97以上。结论本方法具有较好的专属性、精密度和重现性,不仅可增强对极性相似物质的有效分离,用于葛根药材的质量控制,而且其微乳液滴还具有模拟人体生物膜(脂质体)的作用机制,适用于对体内或体外生物样品的分离分析,具有良好的应用前景。
Objective To study and establish the fingerprint of Radix Puerariae by capillary electrophoresis. Methods Microemulsion electrokinetic capillary chromatography. Uncoated fused silica capillary (63.5 cm × 50.0 cm, 50.0 μm) was used. The buffer was a microemulsion with a voltage of 20 kV and a detection wavelength of 254 nm. Results The puerarin peak was taken as the reference peak to establish the microemulsion electrokinetic capillary chromatography fingerprinting of Pueraria lobata. 13 common fingerprint peaks were identified. The components identified by adding sample were daidzin, puerarin, daidzein and Genistein. The similarity of kudzu root fingerprints obtained from three different producing areas and four different extraction methods was evaluated above 0.97. Conclusion The method has good specificity, precision and reproducibility. It can not only enhance the effective separation of polar analogues, but also can be used for the quality control of Radix Puerariae. Moreover, Plastid) mechanism of action, suitable for in vivo or in vitro biological samples of separation and analysis, has a good prospect.