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为了研究中国、意大利、法国、美国等15个国家80份菊苣属种质的遗传多样性及亲缘关系,试验采用SRAP和EST-SSR两种分子标记进行检测。结果表明:SRAP和EST-SSR分别扩增出257条和218条条带,平均多态位点百分率分别为96.2%和99.5%,平均多态信息含量分别为0.320和0.366,平均标记指数分别为7.38和7.15。SRAP检测8个国家菊苣遗传多样性指数均高于ESTSSR检测结果;两种标记检测结果显著相关(r=0.729,P<0.05),结合聚类可将80份材料分为8个组群,来源相同国家的材料基本能聚在一起,但聚类结果与地理分布并不完全一致。说明供试菊苣存在丰富的遗传多样性,其中意大利菊苣种质遗传多样性最丰富;两种标记均能够有效鉴定菊苣种和苦苣种,均适用于菊苣属物种遗传多样性及亲缘关系的鉴定。SRAP标记具有较高的多态性位点检测能力和较高的标记效率。
In order to study the genetic diversity and genetic relationship of 80 chicory genera in 15 countries including China, Italy, France and the United States, SRAP and EST-SSR molecular markers were used to test the genetic diversity. The results showed that 257 and 218 bands were amplified respectively by SRAP and EST-SSR, the average percentage of polymorphic loci was 96.2% and 99.5% respectively, the average polymorphic information content was 0.320 and 0.366, respectively. The average labeling indices were 7.38 and 7.15. The genetic diversity index of chicory in 8 countries was higher than that of ESTSSR by SRAP. The results of two markers were significantly correlated (r = 0.729, P <0.05). According to clustering, 80 materials could be divided into 8 groups, The same country can basically gather together the materials, but the clustering results and geographical distribution are not exactly the same. The result showed that the genetic diversity of the tested chicory was rich, among which the genetic diversity of Italian chicory germplasm was the most abundant. Both markers could effectively identify chicory and abacus species, which were suitable for the identification of genetic diversity and genetic relationship of chicory species . SRAP marker has higher polymorphism locus detection ability and higher labeling efficiency.