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目的：探索一种快速、实用的扩增登革病毒大片段基因的方法。方法：用登革２ 型新几内亚 Ｃ 株（ Ｄ Ｖ２ ， Ｎ Ｇ Ｃ 株）的核酸（ Ｒ Ｎ Ａ） 为模板，研究了在不同条件下（ 病毒和其变性液的量以及ｃ Ｄ Ｎ Ａ 的浓度等） 通过逆转录聚合酶链式反应技术扩增大片段 Ｎ Ｓ３ 基因，并对扩增的目的基因用巢式 Ｐ Ｃ Ｒ 进行了鉴定。结果：用大量的或小量的登革病毒培养上清中提取 Ｒ Ｎ Ａ 的方法在一定条件下均可扩增出大片段 Ｎ Ｓ３ 基因。结论：小量登革病毒提取 Ｒ Ｎ Ａ 扩增大片段 Ｎ Ｓ３ 基因法较大量登革病毒提取 Ｒ Ｎ Ａ 扩增大片段 Ｎ Ｓ３ 基因快速、实用。
Objective: To explore a rapid and practical method of amplifying large fragment of dengue virus. Methods: Nucleic acid (R N A) of dengue type 2 New Guinea C strain (D V2, N G C strain) was used as a template to study the effect of different doses of virus and denaturant and c D N A Concentration, etc.) amplified large fragment of N S3 gene by reverse transcriptionpolymerase chain reaction, and the amplified target gene was identified by nested P C R. Results: Large amount or small amount of dengue virus culture supernatant extracted R N A under certain conditions can amplify a large fragment N S3 gene. Conclusion: A small amount of dengue virus extracted R N A amplified large fragment N S3 gene method than a large number of dengue virus extraction R N A large fragment of N S3 gene amplification quick and practical.