论文部分内容阅读
用卫氏并殖吸虫囊蚴感染大白鼠,检获两种滞育虫体,早期滞育虫体和晚期滞育虫体,用聚丙烯酰胺等电聚焦技术对两种滞育虫体的苹果酸脱氢酶(MDH)的同工酶酶谱进行分析,并与犬体童虫和成虫的酶谱相比较,结果发现在鼠体内从早期滞育虫体到晚期滞育虫体MDH同工酶区带增多,符合“卫氏并殖吸虫的MDH同工酶区带随发育呈增多及复杂的趋势”的研究结果。鼠体的晚期滞育虫体与犬体童虫和成虫的MDH酶谱基本相似,提示卫氏并殖吸虫在转续宿主体内滞育虫体的MDH同工酶的基因表达及修饰过程与适宜宿主体内的虫体可能一致。
Rats were infected with Paragonimus westermanii metacercaria and seized two kinds of larvae, early larvae and late larvae. Polyacrylamide isoelectric focusing technique was used to detect the contents of malate Dehydrogenase (MDH) isoenzymes were analyzed and compared with the zoobenthos and adult zebrafish zymogram, the results showed that in mice from early diapause to late diapause MDH isozymes Increased zone, in line with “Paragonimusi MDH isoenzyme zone with the development of an increasing and complex trend” of the results. The MDH zymogram of late-stage larvae in vivo was similar to that of adult larvae and adult worms, suggesting that the gene expression and modification of MDH isozymes in Paragonimus westermani Host body may be the same body.