【摘 要】
：
Genetic manipulation of human pluripotent stem cells (hPSCs) provides a powerful tool for modeling diseases and developing future medicine.Recently a number of
【机 构】
：
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beij
论文部分内容阅读
Genetic manipulation of human pluripotent stem cells (hPSCs) provides a powerful tool for modeling diseases and developing future medicine.Recently a number of independent genome-editing techniques were developed,including plasmid,bacterial artificial chromosome,adeno-associated virus vector,zinc finger nuclease,transcription activator-like effecter nuclease,and helper-dependent adenoviral vector.Gene editing has been successfully employed in different aspects of stem cell research such as gene correction,mutation knock-in,and establishment of reporter cell lines (Raya et al.,2009;Howden et al.,2011; Li et al.,2011; Liu et al.,2011b;Papapetrou et al.,2011; Sebastiano et al.,2011; Soldner et al.,2011; Zou et al.,2011a).These techniques combined with the utility of hPSCs will significantly influence the area of regenerative medicine.
其他文献
In this review we summarize the progress made towards understanding the role of protein-protein interactions in the function of various bioluminescence systems
The Hippo pathway plays a crucial role in controlling organ size by inhibiting cell proliferation and promoting cell death.Recent findings implicate that this p
考虑到开架借阅所具有的开放性、直观性强的特点，经过对乱架原因的系统分析并结合其它图书馆的经验，我建议采用一种固定架位的模糊排架法（借用模糊控制理论的提法）以取代目前的综
茄子(Solanum melongena L.)起源于亚洲南部热带地区,古印度为其最早的驯化地,中国栽培茄子的历史也很悠久,是茄子的第二起源地,拥有丰富的茄子种质资源。对茄子种质资源分类
Untreated human immunodeficiency virus(HIV)infections usually lead to death from AIDS,although the rate of the disease progression varies widely among individua
After two decades of ups and downs,gene therapy has recently achieved a milestone in treating patients with Leber’s congenital amaurosis (LCA).LCA is a group o
Progranulin(PGRN)has recently emerged as a key player in a subset of frontotemporal dementias(FTD).Numerous mutations in the progranulin gene have been identifi
我国当前作为全世界生产柑橘的第一大国,在柑橘的采后贮藏、运输过程中,常发生各种病原微生物引发的多种病害,导致柑橘的腐烂,并对柑橘产业造成巨大的损失,其中柑橘绿霉病是
In December this year,the 4th Tianqin Cao Memorial Symposium on Protein Research will be held in Xiamen(Amoy).On this occasion,it is my pleasure to write a rec