本文采用纯水作流动相,在一定压力下升高系统温度以保证水处在亚临界状态,FID为检测器,分析了甲醇、异丙醇、正丁醇、2-丁醇、正戊醇、异戊醇、己醇等具有中等以上极性的醇.填充柱(1 mm i.d.)流速50μl@min-1,毛细管柱(50 μm i.d.)流速70 μl.min-1.升高温度至140℃,结合温度梯度,亚临界水可以模拟高效液相色谱常用的甲醇-水混合流动相的极性,可以显著缩短分析时间并改善峰形.分析系统保证了亚临界状态,并有望应用于无紫外吸收、含量极低的极性化合物的分析.“,”A sub-critical chromatography (SubWC) with water as mobile phase and FID detection system isemployed to separate several alcohols with high or medium polarity, with pure water as the eluent. The flow rate getsup to 50 μl@min-1 for packed column (1 mm i.d.) and 70 μl@min-1 for capillary (50 μm i.d.). Increasing the temperatureup to 140 ℃, together with temperature programming, markedly improves the separation and peak shapes withinshort analysis time. Sub-critical state is guaranteed.