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To study retinal S-antigen expression in human retinoblastoma and assess if there is a correlation between S-antigen immunoactivity and degree of retinoblastoma cell differentiations.Methods:Ten cases of Chinese retinoblastoma parafin-embedded tissues were applied for this study. A strain of monoclonal antibody,MabA9C6,which defines an epitope in S-antigen retained in fixed-tissue sections,was used to study S-antigen expression in 10 cases of retinoblastomas. S-antigen was localized by the biotina-vidin indirect immunoperoxidase technique and purified MabA9C6 ascites fluid was used with M1:100 dilution. The whole procedure could be finished within a few hours.Results: The S-antigen immunoactivity was observed in different pattterns: the “normal”photoreceptor elements incorporated in 3 cases of growing tumors;3 of 4 Fleurettes and E-W rosettes;and scattered tumor cells in 50% of the cases. Conclusions: The result suggests that the expression of S-antigen in retinoblastoma may be used to assess the degree
To study retinal S-antigen expression in human retinoblastoma and assess if there is a correlation between S-antigen immunoactivity and degree of retinoblastoma cell differentiations. Methods: Ten cases of Chinese retinoblastoma parafin-embedded tissues were applied for this study. A strain of monoclonal antibody, MabA9C6, which defines an epitope in S-antigen retained in fixed-tissue sections, was used to study S-antigen expression in 10 cases of retinoblastomas. S-antigen was localized by the biotin-vidin indirect immunoperoxidase technique and purified MabA9C6 ascites The whole procedure could be finished within a few hours. Results: The S-antigen immunoactivity was observed in different pattterns: the “normal” photoreceptor elements incorporated in 3 cases of growing tumors; 3 of 4 Fleurettes and EW rosettes; and scattered tumor cells in 50% of the cases. Conclusions: The result suggests that the expression of S-antigen in retinoblastoma may be used to assess the degree