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目的了解慢乙肝患者抗 HBe 阳性和 e 抗原阳性血清的病毒量及不同病变程度的慢乙肝与 HBV 感染量的关系.了解HBV-DNA 含量和血清 ALT 水平之间的关系.方法临床确诊的89例慢乙肝患者,经 ELISA 测定后,选取30例 HBeAg(+)/HBeAb(-)血清和30例 HBeAg(-)/HBeAb(+)血清,采用 AmpliSensor PCR 定量技术,测定 HBV-DNA.使用全自动生化分析仪,测定血清 ALT 水平.各组HBV-DNA 含量和 ALT 水平用求算术平均值的方法计算.结果 HBeAg(+)/HBeAb(-)血清30例,HBV-DNA 平均含量为(11.21±6.92)E 拷贝/L;HBeAg(-)/HBeAb(+)血清22例,HBV-DNA 平均含量为(9.73±5.61)E 拷贝/L,两组具有显著差异(P<0.05).轻度慢性乙肝17例,HBV-DNA 平均含量为(10.93±6.22)E 拷贝/L;中度慢性乙肝23例,HBV-DNA 平均含量为(9.84±6.06)E 拷贝/L;重度慢性乙肝12例,HBV-DNA 平均含量为(8.72±5.62)E 拷贝/L.轻、重度组间 HBV-DNA 平均含量有显著差异(P<0.05).血清 ALT 水平与肝组织病变程度无一致关系,与 HBV-DNA 含量亦无明显相关.结论多数抗-HBe 阳性患者 HBV 仍持续复制,血清病毒量低于 HBeAg(+)者.慢乙肝病变程度与 HBV-DNA 平均拷贝数成反比.肝脏炎症程度似可作为估计病毒量的间接指标.
Objective To investigate the relationship between HBV DNA levels and serum HBV-DNA in patients with chronic hepatitis B (HBeAg-positive and e-antigen positive) and the relationship between serum HBV-DNA levels and HBV-DNA levels.Methods Eighty-nine patients Thirty HBeAg (+) / HBeAb (-) sera and 30 HBeAg (-) / HBeAb (+) sera were collected from patients with chronic hepatitis B. The HBV-DNA was determined by AmpliSensor PCR quantitative assay using ELISA Biochemical analyzer was used to measure the level of serum ALT.The HBV-DNA content and ALT level of each group were calculated by means of arithmetic mean.Results The average content of HBV-DNA in HBeAg (+) / HBeAb (-) serum was (11.21 ± 6.92) E copies / L; HBeAg (-) / HBeAb (+) serum was detected in 22 cases with the mean level of HBV-DNA of 9.73 ± 5.61 copies / L and there was a significant difference between the two groups The average level of HBV-DNA was (10.93 ± 6.22) E copies / L; 23 cases of moderate chronic hepatitis B, the average content of HBV-DNA was (9.84 ± 6.06) E copies / L; 12 cases of severe chronic hepatitis B (8.72 ± 5.62) E copies / L. The mean levels of HBV-DNA in mild and severe groups were significantly different (P <0.05) .The serum levels of ALT and liver tissue lesions There was no consistent relationship between them and HBV-DNA content.Conclusion Most of anti-HBe positive patients continue to replicate HBV, the serum viral load is lower than that of HBeAg (+). The degree of chronic hepatitis B and the average copy number of HBV-DNA Inversely proportional to the extent of liver inflammation can be used as an indirect indicator of the estimated amount of virus.