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利用6-二甲基氨基嘌呤(60μg/cm3;6-DMAP)和细胞松弛素B(0.5μg/cm3;CB)抑制受精卵的第1次卵裂,授精后60和65 min分别用CB和6-DMAP持续处理20和15 min,可诱导出12.5%和27.5%的第1卵裂抑制型雌核发育二倍体.细胞学观察显示,6-DMAP抑制第1次卵裂产生的雌核发育二倍体主要在第1次有丝分裂后期的受精卵,由于破坏了纺锤体和阻止了染色体分离,导致一个二倍性雌核的形成,而CB有效地阻止了第1次卵裂的胞质分裂.尽管倍化率和D型幼虫发生率较低,但该研究首次报道了栉孔扇贝第1卵裂抑制型雌核发育二倍体诱导的可行性.
The first cleavage of fertilized eggs was inhibited by 6-dimethylaminopurine (60μg / cm3; 6-DMAP) and cytochalasin B (0.5μg / cm3; CB) 6-DMAP continued for 20 and 15 min and induced 12.5% and 27.5% of the first cleavage-type gynogenetic diploid. Cytological observations showed that 6-DMAP inhibited the first cleavage of gynogenes Development of diploid fertilized eggs, mainly in the first mitotic period, resulted in the formation of a diploid gland nucleus due to the destruction of the spindle and the prevention of chromosome segregation, whereas CB effectively blocked the cytoplasm of the first cleavage Although the doubling rate and the incidence of D larvae were low, the study reported for the first time the feasibility of diploid induction of the first cleavage-inhibiting gynogenetic scallop Chlamys farreri.