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目的研究丹参酮ⅡA(TSN)对马兜铃酸肾病(AAN)肾间质微血管(PTCs)病变的作用。方法将30只雌性Wister大鼠分为3组:模型组和治疗组各10只,灌胃关木通水煎剂,对照组10只,灌胃饮用水。2h后,治疗组给予丹参酮ⅡA腹腔注射,对照组和模型组注射生理盐水。实验第12周处死全部大鼠,留取血、尿和肾组织标本,分别作生化、病理、免疫组化、免疫印迹、逆转录聚合酶链反应等检查。结果与模型组相比,治疗组大鼠肾组织PTCs密度增加,低氧诱导因子-1α(HIF-1α)表达减少,肾功能损害减轻,尿蛋白减少(P均<0.01)。结论TSN能改善肾脏PTCs病变,保护肾功能,延缓AAN进展。
Objective To investigate the effect of TSN on the pathological changes of renal interstitial capillaries (PTCs) in patients with aristolochic nephropathy (AAN). Methods Thirty female Wister rats were divided into three groups: model group and treatment group, 10 rats in each group were given Guanweidong decoction orally and 10 rats in control group. After 2h, the treatment group was given tanshinone ⅡA intraperitoneal injection, the control group and model group were injected with saline. All the rats were sacrificed on the 12th week of the experiment, blood, urine and kidney tissue samples were collected for biochemical, pathological, immunohistochemical, immunoblot, reverse transcription polymerase chain reaction and other tests. Results Compared with the model group, the density of PTCs, the expression of hypoxia inducible factor - 1α (HIF - 1α), the renal dysfunction and the proteinuria in the kidney of the treated group were decreased (all P <0.01). Conclusion TSN can improve the renal lesions of PTCs, protect renal function and delay the progression of AAN.