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用异硫氰酸 鬼笔环肽 (FITC Ph)标记和共聚焦激光扫描显微镜 (CLSM )观察发现 ,以具有激发子活性的接种叶锈菌的小麦叶片的胞间洗脱液 (IWF)处理叶肉细胞原生质体一定时间后 ,抗病小麦品种洛夫林 10的原生质体内微丝骨架保持完整的网络状结构 ,而感病品种 5 389的大部分微丝骨架处于解聚状态。同时 ,抗病品种因IWF处理诱发的防卫反应———H2 O2 突发和HR反应的程度 (用原生质体活力下降的程度表示 )也大大高于感病品种。用细胞松弛素D(CD)预处理抗病品种原生质体可以明显抑制IWF处理诱发的H2 O2突发和HR反应 ,表明微丝骨架的状态可能与抗病性有密切的关系 ,完整的微丝骨架是H2 O2 突发和HR反应的一个重要条件。
The results of FITC Ph labeling and confocal laser scanning microscopy (CLSM) showed that mesophyll eukaryotic cells (IWF), inoculated with leaf rust, inoculated with leaf rust, After a certain period of time, protoplasts of resistant wheat cultivar Lovrin 10 remained intact network structure, whereas most of the susceptible cultivars 5 389 were in a depolymerized state. At the same time, the extent of the defense response induced by IWF-induced H2O2 burst and HR reaction (expressed by the degree of protoplast viability decline) was also significantly higher than that of susceptible varieties. Pretreatment of resistant varieties with cytochalasin D (CD) protoplasts significantly inhibited the H2O2-induced burst and HR responses induced by IWF treatment, suggesting that the microfilament skeletal status may be closely related to disease resistance, with intact microfilaments The skeleton is an important condition for H2 O2 burst and HR reactions.