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目的探究改良羊水原位培养法是否在产前诊断中具有显著的应用价值。方法对改良羊水原位培养法的各项操作步骤进行系统的分类总结,总结内容主要包括改良羊水采集、改良羊水细胞培养、各类羊水细胞处理以及羊水细胞传代四方面。结果改良羊水原位培养法比之传统的羊水原位培养法,能有效对羊水细胞的收获以及有效性进行干预,显著提升羊水细胞的培养成功率。结论改良羊水原位培养法能够有效提升羊水细胞的培养成功率,对孕产妇的产前诊断具有重大的意义,值得临床推广应用。
Objective To investigate whether improved amniotic fluid in situ culture method has a significant value in prenatal diagnosis. Methods The methods of in situ in situ culture of amniotic fluid were systematically classified and summarized. The main contents of the study include: improving amniotic fluid collection, improving amniotic fluid cell culture, treating amniotic fluid cells and amniocyte passage. Results Improved amniotic fluid in situ culture method than in the traditional method of amniotic fluid in situ culture, the amniotic fluid cells can effectively harvest and the effectiveness of intervention, significantly improve the success rate of amniotic fluid cell culture. Conclusion Improved amniotic fluid in situ culture method can effectively improve the success rate of culture of amniotic fluid cells. It is of great significance to prenatal diagnosis of pregnant women and is worthy of clinical application.