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目的:观察补益营卫方对衰老小鼠皮肤表皮凋亡指数的影响,探讨补益营卫方延缓皮肤衰老的机制。方法:以自然衰老小鼠为研究对象,HE染色观察各组皮肤表皮形态学变化,应用TUNEL法和计算机图像分析系统对凋亡指数表达进行分析。结果:应用补益营卫方高、中、低剂量组处理后,可显著降低细胞凋亡,与老年组比较有统计学意义(P<0.05)。三个剂量组之间,中剂量组对凋亡抑制作用最大,较其他组有明显差异(P<0.01),低剂量组和高剂量组没有明显差异(P>0.05)。结论:补益营卫方通过抑制皮肤表皮细胞的凋亡,降低细胞凋亡指数,起到延缓皮肤衰老作用。
OBJECTIVE: To observe the effect of BuYi YingWeiFang on the apoptosis index of skin epidermis in aging mice and explore the mechanism of BuYiYingWeiDelay on skin aging. Methods: The natural aging mice were used as the research objects. The morphological changes of the skin epidermis of each group were observed by HE staining. The expression of apoptosis index was analyzed by TUNEL method and computer image analysis system. Results: Treated with high, medium and low doses of BuYiYingWei Decoction, the apoptosis was significantly reduced, which was statistically significant compared with the old group (P <0.05). There was no significant difference between low dose group and high dose group (P> 0.05). The inhibitory effect of middle dose group on apoptosis was the highest between the three dose groups (P <0.01). Conclusion: BuYiYingWei Fang can inhibit the skin epidermal cells apoptosis and reduce the apoptosis index, play a role in delaying skin aging.