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青篙素是重要的抗疟药物,青篙素合成量在黄花篙的成花期显著提高。本研究以试管开花为研究体系,探讨黄花篙开花的诱导因子与青篙素生物合成的关系。结果表明:外源细胞分裂素是黄花篙试管开花形成的必需因子,2 mg/L玉米素(ZT)对茎段外植体分化花芽效果最好。在ZT作用下赤霉素(GA3)较显著地提高了黄花篙花芽的分化率。在诱导培养基中只加1/2 NH_4NO_3或不加NH_4NO_3,也有利于花芽的分化和发育。花期植株脱氧木酮糖磷酸合酶(DXS)、脱氧木酮糖磷酸还原酶(DXR)、3-轻基-3-甲基戊二单酞辅酶A还原酶(HMGR)、法呢基焦磷酸合成酶(FPP)基因表达水平显著高于营养期植株,具有成花诱导活性的外源激素组合如ZT、GA3和叫哚丁酸(IBA)可促进植株中青篙素生物合成下游途径中紫穗槐二烯合酶(ADS)和细胞色素P450单氧酶(CYP)基因表达提高青篙素的合成量。本研究为探讨成花物质对青篙素生物合成的影响和利用花器官诱导青篙素高产植株提供了基础和方法。
Bromelain is an important anti-malarial drug, and the synthesis of lutein is significantly increased during the flowering stage. In this study, flowering tube was used as the research system to investigate the relationship between the inducing factor of flowering and the biosynthesis of luteolin. The results showed that exogenous cytokinin was an essential factor for the flowering of Asiatic tuberosas, and 2 mg / L zeatin (ZT) had the best effect on differentiating flower buds of stem explants. Gibberellin (GA3) increased the differentiation rate of flower buds significantly. In the induction medium, only 1/2 NH_4NO_3 or NH_4NO_3 was not added, which also contributed to the differentiation and development of flower buds. Florescence plants were treated with DXS, DXR, HMGR, farnesyl pyrophosphate The expression level of synthase (FPP) gene was significantly higher than that of vegetative plants, and exogenous hormones such as ZT, GA3 and IBA with flowering-inducing activity could promote the downstream pathway of cyanidin biosynthesis in plants The expression of cantharidin synthase was increased by the expression of both locust - forming diene synthase (ADS) and cytochrome P450 monooxygenase (CYP) genes. This study provided the basis and method for investigating the effects of flowering substances on the biosynthesis of luteolin and the use of floral organs to induce high-yielding plants of lutein.