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目的：研究腺病毒介导的多基因（ＧＭ－ＣＳＦ、Ｂ７－１、ｐ５３、ＩＬ－２）对胆管癌细胞系ＱＢＣ９３９ 凋亡的诱导作用和对正常肝细胞的作用。方法：应用ＴＵＮＥＬ细胞凋亡检测法和光镜观察，分析同时含ＧＭ－ＣＳＦ、Ｂ７－１、ｐ５３、ＩＬ－２ ４ 种目的基因的重组腺病毒载体（Ａｄ－ｍ ｕｌｔｉｇｅｎｅｓ）对胆管癌细胞系ＱＢＣ９３９ 和肝细胞系Ｌ０２的作用以及对大鼠肝脏的毒性损害。结果：Ａｄ－ｍｕｌｔｉｇｅｎｅｓ与化疗药物顺铂协同，能诱导３０％ 左右的ＱＢＣ９３９发生凋亡，并对肝细胞系Ｌ０２ 和大鼠肝细胞无明显毒性损害。结论：Ａｄ－ｍ ｕｌｔｉｇｅｎｅｓ能诱导胆管癌细胞ＱＢＣ９３９ 发生凋亡，且与化疗药物顺铂具有协同增强作用。初步分析对人肝细胞及大鼠肝脏无明显毒性损害，具有一定的临床应用前景。
AIM: To investigate the effects of adenovirus-mediated multiple genes (GM-CSF, B7-1, p53 and IL-2) on the apoptosis of cholangiocarcinoma cell line QBC939 and its effect on normal hepatocytes. Methods: TUNEL cells apoptosis assay and light microscopy were used to analyze the effect of Ad-m ultigenes containing four genes of GM-CSF, B7-1, p53 and IL-2 on the cell proliferation of cholangiocarcinoma cell line QBC939 And the role of the hepatocyte cell line L02 as well as toxic damage to rat livers. Results: Ad-multigenes and cisplatin combined with chemotherapeutic drugs could induce about 30% apoptosis of QBC939 and no obvious toxic damage to L02 cells and rat hepatocytes. Conclusion: Ad-m ultigenes can induce the apoptosis of human cholangiocarcinoma cell line QBC939 and has synergistic effect with chemotherapeutic drug cisplatin. Preliminary analysis of human liver cells and rat liver no significant toxicity, with some clinical application prospects.