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随着基因工程技术的广泛应用,迫切需要有高效表达的质粒载体,为此,我们对含Ptrc启动子的大肠杆菌表达质粒pkk 233-2进行改建,使其更适于表达外源基因,并在大肠杆菌高效表达成功Cat(氯霉素乙酰转酰基酶)和小鼠白细胞介素-2(IL-2)。
With the wide application of genetic engineering techniques, there is an urgent need for a highly efficient plasmid vector. Therefore, we reconstructed the E. coli expression plasmid pkk 233-2 containing the Ptrc promoter to make it more suitable for the expression of foreign genes Cat (chloramphenicol acetyl transacylase) and mouse interleukin-2 (IL-2) were successfully expressed in E. coli.