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目的:探讨维生素E对锰中毒小鼠学习记忆及海马区caspase-3表达的影响。方法:将60只雄性昆明小鼠随机分为四组:对照组(CG)、维生素E组(VG)、锰中毒组(MG)、锰中毒加维生素E组(MVG),每组15只。采用腹腔注射氯化锰的方式制作锰中毒模型,Morris水迷宫观察小鼠的学习记忆能力,免疫组织化学方法检测caspase-3蛋白的表达。结果:Morris水迷宫定位航行实验显示,CG、VG、MVG三组的逃避潜伏期比MG组明显缩短,空间探索实验中,CG、VG、MVG三组穿越平台的次数明显多于MG组。免疫组织化学实验中,MG组海马CA1区和DG区caspase-3的表达明显多于CG组,MVG组海马区caspase-3的阳性细胞数明显少于MG组。结论:维生素E干预可减少海马区神经细胞的凋亡,改善锰中毒小鼠的学习记忆能力。
Objective: To investigate the effect of vitamin E on learning and memory and the expression of caspase-3 in hippocampus of mice with manganese poisoning. Methods: Sixty male Kunming mice were randomly divided into four groups: control group (CG), vitamin E group (MG), manganese poisoning group and MVG group (n = 15). Manganese poisoning model was made by intraperitoneal injection of manganese chloride. The learning and memory ability of mice was observed by Morris water maze and the expression of caspase-3 protein was detected by immunohistochemistry. Results: Morris water maze positioning navigation experiment showed that the escape latency of CG, VG and MVG groups was significantly shorter than that of MG group. In space exploration experiments, CG, VG and MVG groups crossed the platform significantly more than MG group. In immunohistochemistry, the expression of caspase-3 in CA1 and DG of hippocampus in MG group was significantly higher than that in CG group. The number of caspase-3 positive cells in hippocampus of MVG group was significantly less than that in MG group. Conclusion: Vitamin E can reduce the apoptosis of neurons in hippocampus and improve learning and memory in mice with manganese poisoning.