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目的探讨重组慢病毒介导的人核小体结合蛋白1(NSBP1)基因沉默对非激素依赖性前列腺癌细胞系DU145凋亡和增殖的调控机制。方法慢病毒lentivirus-shRNA-NSBP1转染非激素依赖性前列腺癌细胞系DU145,MTT法检测细胞生长活性,流式细胞技术检测细胞凋亡和细胞周期。Western blot方法检测敲减NSBP1蛋白后细胞中cyclinB1与BCL-2蛋白的表达。结果体外实验证实NSBP1表达水平的降低,对肿瘤细胞的生长有明显抑制作用,96h细胞生长抑制率为22.6%。同时随着NSBP1表达水平的降低,cyclinB1和BCL-2的蛋白的表达也降低。结论 NSBP1-RNAi-lentivirus重组慢病毒能有效抑制DU145细胞的生长,NSBP1可能通过调控cyclinB1、BCL-2基因的变化影响肿瘤细胞的生长。
Objective To investigate the regulatory mechanism of recombinant lentivirus-mediated silencing of nucleosome-binding protein 1 (NSBP1) on the apoptosis and proliferation of non-hormone-dependent prostate cancer cell line DU145. Methods Lentivirus lentivirus-shRNA-NSBP1 was transfected into DU145, a hormone-independent prostate cancer cell line. The cell growth activity was measured by MTT assay. Apoptosis and cell cycle were detected by flow cytometry. Western blot was used to detect the expression of cyclinB1 and BCL-2 in NSBP1 knockdown cells. Results In vitro experiments confirmed that NSBP1 expression decreased, the growth of tumor cells were significantly inhibited 96h cell growth inhibition rate was 22.6%. Meanwhile, with the decrease of NSBP1 expression, the expression of cyclinB1 and BCL-2 proteins also decreased. Conclusion NSBP1-RNAi-lentivirus recombinant lentivirus can effectively inhibit the growth of DU145 cells. NSBP1 may affect the growth of tumor cells by regulating the changes of cyclinB1 and BCL-2 genes.