目的　探讨地塞米松、氨甲喋呤对人眼球后成纤维细胞增殖的影响。方法　采用体外培养人眼球后成纤维细胞(HROFb) ,用MTT比色法测定HROFb增殖程度 ,并观察了不同浓度地塞米松、氨甲喋呤单独及联合使用对HROFb生长、增殖的影响。结果和结论　MTT比色法能精确反映HROFb的增殖程度 ,所测定吸收值与细胞数目成正相关 (r =0 .996 ,P <0 .0 0 1) ,地塞米松、氨甲喋呤均可抑制HROFb的增殖 ,且随剂量加大 ,抑制作用增强 ,两者联合使用可减少用量 ,增加抑制作用。 Objective To investigate the effects of dexamethasone and methotrexate on the proliferation of human ocular fibroblasts. Methods Human eyeball fibroblasts (HROFb) were cultured in vitro. The proliferation of HROFb was measured by MTT colorimetric assay. The effects of different concentrations of dexamethasone and methotrexate alone or in combination on the growth and proliferation of HROFb were observed. RESULTS AND CONCLUSION MTT colorimetric method could accurately reflect the proliferation of HROFb. The measured absorbance was positively correlated with cell number (r = 0.996, P <0.01). Dexamethasone and methotrexate could inhibit the growth of HROFb Proliferation, and with the dose increased, the inhibitory effect increased, the combination of the two can reduce the dosage and increase inhibition.