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筛选一种快速、准确的测定肠球菌对高浓度的庆大霉素、链霉素的耐药性的方法。分别用单一浓度肉汤微量稀释法、ＶＩＴＥＫ－ＧＰＳ－ＴＡ药敏卡检测１６３株肠球菌对庆大霉素（５００ｍｇ／Ｌ，２０００ｍｇ／Ｌ）、链霉素（２０００ｍｇ／Ｌ）的耐药性，并以脑心浸液方法为标准，对上述两种方法的准确性进行评价。显示，单一浓度肉汤微量稀释法能准确地测定肠球菌对庆大霉素的高度耐药性，但在测定对高浓度的链霉素的耐药性时，类粪肠球菌，鸟肠球菌有个别假敏感株出现。ＶＩＴＥＫ－ＧＰＳ－ＴＡ药敏卡能准确测定粪肠球菌、鸟肠球菌对庆大霉素的高度耐药性，但在测定类粪肠球菌、鸟肠球菌对庆大霉素及全部肠球菌对链霉素的耐药性时均有假敏感株出现。单一浓度肉汤微量稀释法能较准确地测定肠球菌对高浓度氨基糖甙类抗生素的耐药性，而ＶＩＴＥＫ－ＧＰＳ－ＴＡ药敏卡则不适宜高度耐药株的筛选。
Screening a rapid and accurate method for the determination of Enterococcus high concentrations of gentamicin, streptomycin resistance method. The drug resistance of 163 enterococci to gentamycin (500mg / L, 2000mg / L) and streptomycin (2000mg / L) was tested by single dilution broth microdilution method and VITEK-GPS- , And brain and heart infusion method as the standard, the accuracy of these two methods were evaluated. Showed that a single concentration of broth microdilution method can accurately determine the high resistance of enterococci to gentamicin, but in the determination of high concentrations of streptomycin resistance, Enterococcus faecalis, Enterococcus faecalis There are several false-sensitive strains appear. VITEK-GPS-TA drug sensitive card can accurately detect Enterococcus faecalis, Enterococcus faecium highly resistant to gentamicin, but in the determination of Enterococcus faecalis, Enterococcus faecium gentamicin and all enterococci pairs Streptomycin resistance when there are false-sensitive strains appear. Single-concentration broth microdilution method can be more accurately measured enterococci high concentrations of aminoglycoside antibiotic resistance, and VITEK-GPS-TA susceptibility card is not suitable for screening highly resistant strains.