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目的:为确证2010年浙江省输入性疑似登革热病例病因,分析病原的分子特征并进行溯源。方法:对患者血清标本分别检测登革IgM抗体、病毒核酸和病毒分离。对分离株E和NS1基因测序,并进行同源性和进化分析。结果:从血清中检测到登革IgM抗体和登革2型核酸,并分离到登革2型病毒株(ZJ/02/10);浙江登革2型分离株与标准株NGC在E基因的核苷酸(nt)和氨基酸(aa)同源性分别为94.7%和97.6%,而与登革1、3、4型的nt同源性分别为65.3%、64.3%和65.9%。ZJ/02/10株与菲律宾PHI/St68/00株E基因nt和aa同源性最高,进化树显示二者亲缘关系最近。NS1基因进化树结果与E基因相似。结论:从血清学、病原学和分子特征均证实输入性疑似登革病例由登革2型病毒引起,该毒株最有可能来源于菲律宾。
Objective: To confirm the etiological factor of suspected imported dengue cases in Zhejiang province in 2010, the molecular characteristics of pathogens were analyzed and traced back. Methods: The dengue IgM antibody, virus nucleic acid and virus were isolated from the serum samples of patients. The isolate E and NS1 genes were sequenced and homology and evolution analysis was performed. Results: Dengue IgM antibody and dengue 2 nucleic acid were detected in serum, and dengue 2 type virus (ZJ / 02/10) was isolated. Zhejiang Dengue 2 isolates and NGC The nucleotide (nt) and amino acid (aa) homologies were 94.7% and 97.6%, respectively. The nt homologies with dengue 1,3,4 were 65.3%, 64.3% and 65.9%, respectively. ZJ / 02/10 and PHI / St68 / 00 strains of Philippines have the highest homology of nt and aa, and the phylogenetic tree shows the closest relationship between them. The NS1 phylogenetic tree is similar to the E gene. CONCLUSIONS: The dengue 2-type enterovirus-positive cases of imported suspected dengue cases were confirmed from serological, etiological and molecular features, most likely from the Philippines.