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目的:研究补肾方对去势后雌鼠骨质疏松的治疗及其机制。方法:80只雌性SD大鼠分为5组:正常对照组、假手术组、骨质疏松模型组、雌激素组、补肾方高、低剂量组。手术12周后开始给药,连续给药12周,放射免疫法检测血清雌激素(E2)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和胰岛素样生长因子-1(IGF-1)含量,观察股骨组织的病理变化。结果:模型组E2,IGF-1含量下降;补肾方低剂量组和雌激素组E2,IGF-1含量显著增加,IL-6和TNF-α含量显著下降。补肾方低剂量组与雌激素组比较差异无显著性。结论:补肾中药有明显抗去势大鼠骨质疏松的作用,其机制可能与增加E2和IGF-1、降低IL-6和TNF-α水平有关,为临床应用补肾中药治疗绝经后骨质疏松提供依据。
Objective: To study the therapeutic effect of Bushen recipe on osteoporosis in ovariectomized female rats and its mechanism. Methods: Eighty female SD rats were divided into five groups: normal control group, sham operation group, osteoporosis model group, estrogen group and Bushen Fang high and low dose groups. After 12 weeks of operation, administration was continued for 12 weeks. Serum estrogen (E2), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and insulin-like growth Factor-1 (IGF-1) content, observe the pathological changes of femur. Results: The content of E2 and IGF-1 in the model group decreased. The content of E2 and IGF-1 in the Bushenfang low dose group and the estrogen group were significantly increased, and the contents of IL-6 and TNF-α were significantly decreased. Bushenfang low dose group and estrogen group no significant difference. Conclusion: Bushen Chinese medicine has obvious anti-castration rat osteoporosis, the mechanism may be related to increasing E2 and IGF-1, reducing the level of IL-6 and TNF-α, for the clinical application of traditional Chinese medicine in the treatment of postmenopausal osteoporosis Provide evidence.