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目的研究全反式维甲酸（ＡＴＲＡ）对人肝细胞癌细胞ＢＥＬ７４０４细胞生长的影响。方法藉ＭＴＴ法观察ＡＴＲＡ（１０－５ｍｏｌ／Ｌ）对ＢＥＬ７４０４细胞的生长及其对人表皮生长因子刺激的反应性的影响。结果ＡＴＲＡ可使ＢＥＬ７４０４细胞生长抑制，１０－５ｍｏｌ／ＬＡＴＲＡ作用５天，细胞生长抑制率为６０％。经ＡＴＲＡ预处理５天后的细胞，换不含ＡＴＲＡ的培养液，在第５天生长抑制率为２２％（Ｐ＜０．０１），且其对人表皮生长因子促增殖作用的反应性显著降低，表皮生长因子作用的细胞增殖率，对照组为１７７．９％±１．０％（７２ｈ），ＡＴＲＡ预处理组为１６２．５％±２．３％（７２ｈ）（Ｐ＜０．０１）。结论ＡＴＲＡ可能藉降低细胞对表皮生长因子刺激的反应性抑制人肝细胞癌细胞的增殖。
Objective To investigate the effect of all-trans retinoic acid (ATRA) on the growth of human hepatocellular carcinoma cell line BEL7404. Methods MTT assay was used to observe the effect of ATRA (10-5 mol/L) on the growth of BEL7404 cells and its response to human epidermal growth factor stimulation. RESULTS: ATRA inhibited the growth of BEL7404 cells, and the effect of 10-5 mol/LATRA for 5 days showed a 60% inhibition of cell growth. After 5 days of ATRA-pretreatment, cells without ATRA had a growth inhibition rate of 22% on the 5th day (P<0.01), and their reactivity to human epidermal growth factor was significantly reduced. The proliferation rate of cells treated with epidermal growth factor was 177.9%±1.0% (72 hours) in the control group and 162.5%±2.3% (72 hours) in the ATRA pretreatment group (P<0.01). . Conclusion ATRA may inhibit the proliferation of human hepatocellular carcinoma cells by reducing the response of cells to epidermal growth factor stimulation.