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目的 观察胃癌组织中p2 7基因的表达和增殖细胞核抗原(PCNA)染色强度指数间的关系及临床意义。方法 采用LSAB免疫组织化学法。结果 在68例原发性胃癌组织中,p2 7基因阳性表达率47 0 6% (3 2 /68) ,PCNA染色指数平均为5 9 18±3 2 8,p2 7基因阳性率和PCNA染色指数在组织学分级Ⅰ级中明显低于组织学分级Ⅲ级(P <0 0 1) ;在无淋巴结转移者中明显低于有淋巴结转移者(P <0 0 5 )。结论 胃癌组织中p2 7基因的表达和PCNA染色指数与胃癌的发生、发展密切相关,可以客观反映胃癌的组织分化状态,肿瘤进展及转移潜力。
Objective To observe the relationship between the expression of p27 gene and the staining intensity index of proliferating cell nuclear antigen (PCNA) in gastric carcinoma and its clinical significance. Methods Using LSAB immunohistochemistry. Results The positive rate of p27 gene in 68 cases of primary gastric cancer was 47 0 6% (3 2/68), the average of PCNA staining index was 5 9 18 ± 3 2 8, the positive rate of p27 and PCNA staining index The histological grade Ⅰ was significantly lower than the histological grade Ⅲ (P <0.01), and was significantly lower than those with lymph node metastasis (P <0 05) in those without lymph node metastasis. Conclusion The expression of p27 gene and PCNA staining index in gastric cancer are closely related to the occurrence and development of gastric cancer, and can objectively reflect the tissue differentiation status, tumor progression and metastasis potential of gastric cancer.